The recalcitrant plant species, Castanospermum australe and Trichilia dregeana, differ in their ability to produce dehydrin-related polypeptides during seed maturation and in response to ABA or water-deficit-related stresses
The recalcitrant plant species, Castanospermum australe and Trichilia dregeana, differ in their ability to produce dehydrin-related polypeptides during seed maturation and in response to ABA or water-deficit-related stresses
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DOI：
10.1093/jexbot/48.314.1717
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发表时间：
1997-09-01
影响因子：
6.9
通讯作者：
Kermode, AR
中科院分区：
文献类型：
--
作者：
Han, B;Berjak, P;Kermode, AR
在干旱胁迫下，柠檬属植物的种子在发育过程中不能获得脱水耐性，在高含水量时会从母体脱落。研究了柠檬属植物种子在发育和萌发过程中对脱落酸（阿坝）的反应，以及在各种水分亏缺相关胁迫（包括脱水、水分胁迫、高盐、高渗透压和低温。两个热带物种表现出不同的能力，在种子成熟过程中产生的蛋白质。南方栗树种子成熟中期和成熟期的胚轴和子叶中均存在脱水蛋白。在Trichilia dregeana中，在成熟种子中未检测到与Bclaim相关的多肽。在C. australe种子中，子叶和轴中积累的甜菜碱相关多肽的性质发生变化，并且在成熟种子中检测到在成熟中期不存在的新多肽。成熟种子的子叶中存在的甜菜碱蛋白（31、37和40 kDa）在萌发后（即在未处理的幼苗中）仍然是可检测的。这些蛋白质在C.在阿坝和除冷以外的所有胁迫处理后，尽管大多数的甜菜碱蛋白仍然是可检测的，但干燥处理的幼苗除外，其中没有检测到甜菜碱蛋白。在C. australe幼苗，发芽后没有发现阿坝或施加于幼苗的任何胁迫处理在根中也没有诱导它们。在阿坝或水分亏缺胁迫下，毛蕊花幼苗的根或子叶中不产生甜菜碱。
In constrast to seeds of orthodox species, those of recalcitrant species do not acquire desiccation tolerance during their development and are shed from the parent plant at high water contents, Dehydrin production in seeds of recalcitrant species was examined during development and germination, in response to abscisic acid (ABA), and following the imposition of various water-deficit-related stresses, including desiccation, water stress, high salt, high osmolarity, and low temperature. Two tropical species exhibited a differential capacity to produce dehydrin-related proteins during seed maturation. Dehydrins were present in axes and cotyledons of Castanospermum australe seeds during mid-maturation and at maturity. In Trichilia dregeana, no dehydrin-related polypeptides were detected in the mature seed. During the development of C. australe seeds, the nature of the dehydrin-related polypeptides accumulated in the cotyledons and axis changed and new polypeptides were detected in the mature seeds that were not present during mid-maturation. The dehydrins present in cotyledons of mature seeds (31, 37 and 40 kDa) were still detectable after germination (i.e. in untreated seedlings). These dehydrins became less abundant in the cotyledons of C. australe seedlings following ABA and all stress treatments except cold, although most of the dehydrins were still detectable, An exception was the desiccation-treated seedlings, in which no dehydrins were detected. In the roots of C. australe seedlings, no dehydrins were found after germination nor were they induced in the root by ABA or any of the stress treatments imposed on seedlings. Seedlings of Trichilia dregeana did not produce dehydrins in the roots or cotyledons when exposed to ABA or water-deficit-related stresses.