Molecular assembly of superquenchers in signaling molecular interactions
Molecular assembly of superquenchers in signaling molecular interactions
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DOI：
10.1021/ja053482t
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发表时间：
2005-09-21
影响因子：
15
通讯作者：
Tan, WH
中科院分区：
文献类型：
--
作者：
Yang, CYJ;Lin, H;Tan, WH
我们设计了一种新型的猝灭剂分子组装，以形成具有优异猝灭效率的超猝灭剂。通过组装不同类型和不同数量的淬灭剂分子，可以根据需要设计超淬灭剂。通过将超猝灭剂标记到分子信标上，获得了320倍的荧光信号增强，而使用相同单体猝灭剂制备的分子信标的荧光信号增强了约14倍。我们的分子组装方法可以有效地提高各种荧光分析的灵敏度，并可广泛用于分子相互作用研究。
We have designed a novel molecular assembly of quencher molecules to form superquenchers with excellent quenching efficiency. The superquencher can be engineered as desired by assembling different types and different numbers of quencher molecules. By labeling a superquencher to a molecular beacon, a 320-fold enhancement of fluorescent signal was achieved, compared to about 14-fold from a molecular beacon prepared with the same monomer quencher. Our molecular assembly approach can effectively improve the sensitivity of a variety of fluorescent assays and can be widely useful for molecular interaction studies.