Heparin-Engineered Mesoporous Iron Metal-Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers
Heparin-Engineered Mesoporous Iron Metal-Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers
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DOI：
10.1002/adhm.201400755
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发表时间：
2015-06-03
影响因子：
10
通讯作者：
Horcajada, Patricia
中科院分区：
文献类型：
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作者：
Bellido, Elena;Hidalgo, Tania;Horcajada, Patricia
有前途的多孔金属有机框架纳米载体（nanoMOFs）的外表面的特定改性保持其特征孔隙率仍然是一个重大挑战。在这里，一个简单，快速，和biofriendly的基准介孔铁（III）trimesate纳米粒子MIL-100（Fe）与肝素，与较长的血液循环时间相关的生物聚合物的外部功能化的方法报告。首先，包覆的纳米颗粒显示出完整的晶体结构和多孔性，在模拟生理条件下具有改善的胶体稳定性，此外还保留了其包封和控释能力。通过细胞摄取、细胞毒性、氧化应激、细胞因子产生、补体激活和蛋白质吸附分析来评价肝素涂层对nanoMOF与生物环境相互作用的影响。这些结果证实，肝素涂层赋予纳米MOF改善的生物学特性，例如减少的细胞识别、缺乏补体激活和活性氧物质产生。总的来说，使用简单而直接的方法涂覆纳米MOF表面的能力可以被视为增强多功能性的一种方式，从而增强多孔MOF纳米颗粒在生物医学中的潜力。
The specific modification of the outer surface of the promising porous metal-organic framework nanocarriers (nanoMOFs) preserving their characteristic porosity is still a major challenge. Here a simple, fast, and biofriendly method for the external functionalization of the benchmarked mesoporous iron(III) trimesate nanoparticles MIL-100(Fe) with heparin, a biopolymer associated with longer-blood circulation times is reported. First, the coated nanoparticles showed intact crystalline structure and porosity with improved colloidal stability under simulated physiological conditions, preserving in addition its encapsulation and controlled release capacities. The effect of the heparin coating on the nanoMOF interactions with the biological environment is evaluated through cell uptake, cytotoxicity, oxidative stress, cytokine production, complement activation, and protein adsorption analysis. These results confirmed that the heparin coating endowed the nanoMOFs with improved biological properties, such as reduced cell recognition, lack of complement activation, and reactive oxygen species production. Overall, the ability to coat the surface of the nanoMOFs using a simple and straight-forward approach could be taken as a way to enhance the versatility and, thus, the potential of porous MOF nanoparticles in biomedicine.