Bioassay of Glutathione S-transferase Antibody-Antigen Interactions Using Microcantilever Sensor
Bioassay of Glutathione S-transferase Antibody-Antigen Interactions Using Microcantilever Sensor
复制标题
使用微悬臂梁传感器对谷胱甘肽 S-转移酶抗体-抗原相互作用进行生物测定
DOI：
10.14233/ajchem.2013.oh44
复制
发表时间：
2013
影响因子：
--
通讯作者：
Zhang, Mingxu
中科院分区：
文献类型：
--
作者：
Wu, Xiuju;Wang, Rui;Lv, Zhili;Zhang, Mingxu
蛋白质调控的表现是细胞中大部分的生化过程，其检测的基本应用范围从临床诊断、环境控制到国土安全问题。同时，研究蛋白质是一项复杂而具有挑战性的任务。历史上，广泛使用的用于蛋白质检测的生物化学方法，例如蛋白质印迹和酶联免疫吸附测定，通常是耗时和材料消耗的，并且需要多步骤方案。其他（例如荧光）需要附着标签。生物物理传感器，如表面等离子体共振和石英晶体微天平，提供了几个优点，如实时，无标记检测。近年来，出现了非常有利的方法是微悬臂梁传感器。在过去的10年中，悬臂梁传感器的应用扩展到溶液中的生物化合物的测量，从而产生了多功能的生物传感器。由于其无标记检测原理和体积小，这种生物传感器有利于诊断应用，疾病监测和基因组学或蛋白质组学研究。生化物质吸附在微悬臂梁的功能化表面上会引起表面应力，从而引起悬臂梁弯曲。它已成功地应用于气敏领域，在基因组学和蛋白质组学。本文研制了一种抗GST抗体的微悬臂梁免疫传感器。用微悬臂梁传感器测定谷胱甘肽S-转移酶抗体-抗原相互作用
The performance of proteins regulation is most of the biochemical processes in cells and their detection essential applications ranging from clinical diagnosis, environmental control to homeland security issues. At the same time it is a complex and challenging task to study proteins. Historically, widely used biochemical methods for protein detection, such as Western blotting and enzyme-linked immunosorbent assay, were usually time and material consuming and require multistep protocols. Others (e.g. fluorescence) require the attachment of labels. Biophysical sensors, such as surface plasmon resonance and quartz crystal microbalance, offer several advantages, such as real-time, label-free detection. Recently, emerged highly advantageous method is the microcantilever sensor. Over the last 10 years, the application of the cantilever sensor was extended to the measurements of bio-compounds in solution, resulting in a versatile biosensors. Because of its label-free detection principle and small size, this kind of biosensor is advantageous for diagnostic applications, disease monitoring and research in genomics or proteomics. The adsorption of biochemical species on a functionalized surface of a microfabricated cantilever can cause surface stress and consequently induce cantilever bending. It has been successfully applied in the gas-sensing field, in genomics and proteomics. In the present study, a microcantilever immunosensor for anti-GST antibody for was developed. Glutathione S-transferase Bioassay of Glutathione S-transferase Antibody-Antigen Interactions Using Microcantilever Sensor†