A gold nanocluster chemical tongue sensor array for Alzheimer's disease diagnosis
A gold nanocluster chemical tongue sensor array for Alzheimer's disease diagnosis
复制标题
用于诊断阿尔茨海默病的金纳米簇化学舌传感器阵列
DOI：
10.1016/j.colsurfb.2018.10.020
复制
发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Han, Xiaojuan;Man, Zhentao;Li, Wei
阿尔茨海默病（Alzheimer's disease，AD）是老年人常见的神经退行性疾病，给社会带来了沉重的经济负担。使用血清学生物标志物是诊断AD的一种有吸引力的方法。虽然在许多研究中已经探索了基于血液的AD生物标志物的测定，但很少有实用的诊断方法用于临床。在这项工作中，我们构建了一个"化学舌"传感器阵列，易于使用，并基于四种荧光金纳米团簇（Au NC）在纳摩尔浓度下区分多种蛋白质。该装置利用基于荧光强度响应模式的线性判别分析。使用这种化学舌头传感器阵列，即使在复杂的生物系统中，如人类尿液中，也可以自信地识别多种蛋白质。最重要的是，AD患者的血清可以有效地与骨关节炎患者或健康人的血清区分开。此外，通过CSF测定验证了通过化学舌传感器阵列针对AD患者获得的结果。我们的结论是，在这项工作中制造的化学舌传感器阵列铺平了道路，设计一种辅助诊断方法，为AD，是侵入性较小，更方便的大规模筛选患者。
Alzheimer's disease (AD) is a common neurodegenerative disorder in elderly people, and is associated with a heavy financial burden on our society. The use of serologic biomarkers is an attractive method to diagnose AD. Although the determination of blood-based biomarkers for AD has been explored in many studies, few practical diagnosis methods have been used in the clinic. In this work, we constructed a "chemical tongue" sensor array that is easy to use and based on four kinds of fluorescent gold nanoclusters (Au NCs) for discriminating between multiple proteins at nanomolar concentrations. The device utilizes a linear discrimination analysis based on fluorescence intensity response patterns. Using this chemical tongue sensor array, multiple proteins can be confidently identified even in complex biological systems, such as human urine. Most importantly, sera of AD patients could be effectively discriminated from those of osteoarthritis patients, or of healthy people. Also, the results obtained for the AD patients by the chemical tongue sensor array were validated by CSF determination. We conclude that the chemical tongue sensor array manufactured in this work paves the way for designing an auxiliary diagnosis method for AD that is less invasive and more convenient for the large-scale screening of patients.