THE CLEAVABLE PRE-SEQUENCE OF AN IMPORTED CHLOROPLAST PROTEIN DIRECTS ATTACHED POLYPEPTIDES INTO YEAST MITOCHONDRIA
THE CLEAVABLE PRE-SEQUENCE OF AN IMPORTED CHLOROPLAST PROTEIN DIRECTS ATTACHED POLYPEPTIDES INTO YEAST MITOCHONDRIA
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DOI：
10.1002/j.1460-2075.1986.tb04365.x
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发表时间：
1986-06-01
期刊：
影响因子：
11.4
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
HURT, EC;SOLTANIFAR, N;SCHATZ, G
输入叶绿体和线粒体蛋白的可切割前序列有几个共同特征。这种结构相似性促使我们测试叶绿体前序列（“转运肽”）是否也可以由线粒体输入机制进行编码。在绿藻莱茵衣藻中，核酮糖-1,5-二磷酸羧化酶/加氧酶 (Rubisco)（一种叶绿体蛋白）的小亚基在细胞质中进行核编码和合成，具有 45 个残基的瞬时前序列。该叶绿体前序列的 31 个氨基末端残基与小鼠二氢叶酸还原酶（一种胞质蛋白）和细胞色素氧化酶亚基 IV（一种输入的线粒体蛋白）融合，其中真实的前序列已被去除。叶绿体前序列将两种附着的蛋白质转运到酵母线粒体基质或内膜中，尽管它的功能不如真正的线粒体前序列有效。我们得出结论，线粒体和叶绿体前序列通过类似的机制发挥其功能。
The cleavable pre-sequences of imported chloroplast and mitochondrial protins have several features in common. This structural similarity prompted us to test whether a chloroplast pre-sequence (''transit peptide'') can also be dtcoded by the mitochondrial import machinery. In the green alga, Chlamydomonas reinhardtii, the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (a chloroplast protein) is nuclear-encoded and synthesized in the cytosol with a transient pre-sequence of 45 residues. The 31 amino-terminal residues of this chloroplast pre-sequence were fused to mouse dihydrofolate reductase (a cystolic protein) and to yest cytochrome oxidase subunit IV (an imported mitochondrial protein) from which the authentic pre-sequence had been removed. The chloroplast pre-sequence transported both attached proteins into the yeast mitochondrial matrix or inner membrane, although it functioned less efficiently than an authentic mitochondrial pre-sequence. We conclude that mitochondrial and chloroplast pre-sequences perform their function by a similar mechanism.