An NCAM mimetic, FGL, alters hippocampal cellular morphometry in young adult (4 month-old) rats.
An NCAM mimetic, FGL, alters hippocampal cellular morphometry in young adult (4 month-old) rats.
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NCAM 模拟物 FGL 可以改变年轻成年（4 个月大）大鼠的海马细胞形态。
DOI：
10.1007/s11064-012-0908-9
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发表时间：
2013
影响因子：
4.4
通讯作者：
Ojo B
中科院分区：
文献类型：
--
作者：
Ojo B
神经细胞粘附分子（NCAM）在CNS中广泛表达，并在发育、认知、神经可塑性和免疫系统调节中发挥作用。因此，NCAM是治疗脑疾病的潜在重要药理学靶点。一种细胞粘附模拟物FGL，一种衍生自NCAM的第二纤连蛋白III型模块的15个氨基酸的肽，已被证明在实验性疾病和衰老模型中充当神经保护剂，恢复海马/认知功能并显著减轻CNS中的有害变化。然而，FGL对年轻健康大鼠海马的影响尚不清楚。本研究探讨了皮下注射FGL对年轻（4个月大）大鼠海马细胞形态学的细胞神经生物学后果。我们确定了FGL对海马体积、锥体神经元数量/密度的影响（使用无偏定量体视学），并检查了神经发生的各个方面（使用2D形态学分析）。FGL处理减少背海马的总体积（与CA 1和CA 3中锥体神经元总数减少相关），并增加齿状回中双皮质素免疫标记神经元的数量，表明可能对年轻健康大鼠的神经发生产生影响。这些数据表明，FGL对海马具有特定的年龄依赖性作用，根据CNS的发育和成熟而不同。
The neural cell adhesion molecule, NCAM, is ubiquitously expressed within the CNS and has roles in development, cognition, neural plasticity and regulation of the immune system. NCAM is thus potentially an important pharmacological target for treatment of brain diseases. A cell adhesion mimetic FGL, a 15 amino-acid peptide derived from the second fibronectin type-III module of NCAM, has been shown to act as a neuroprotective agent in experimental disease and ageing models, restoring hippocampal/cognitive function and markedly alleviating deleterious changes in the CNS. However, the effects of FGL on the hippocampus of young healthy rats are unknown. The present study has examined the cellular neurobiological consequences of subcutaneous injections of FGL, on hippocampal cell morphometry in young (4 month-old) rats. We determined the effects of FGL on hippocampal volume, pyramidal neuron number/density (using unbiased quantitative stereology), and examined aspects of neurogenesis (using 2D morphometric analyses). FGL treatment reduced total volume of the dorsal hippocampus (associated with a decrease in total pyramidal neuron numbers in CA1 and CA3), and elevated the number of doublecortin immunolabeled neurons in the dentate gyrus, indicating a likely influence on neurogenesis in young healthy rats. These data indicate that FGL has a specific age dependent effect on the hippocampus, differing according to the development and maturity of the CNS.