QUANTITATIVE IMMUNOHISTOCHEMICAL DETERMINATION OF CATHEPSIN-D AND ITS RELATION WITH OTHER VARIABLES
QUANTITATIVE IMMUNOHISTOCHEMICAL DETERMINATION OF CATHEPSIN-D AND ITS RELATION WITH OTHER VARIABLES
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DOI：
10.1007/bf00682695
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发表时间：
1993-01-01
影响因子：
3.8
通讯作者：
SILVESTRINI, R
中科院分区：
文献类型：
--
作者：
VENERONI, S;DAIDONE, MG;SILVESTRINI, R
采用免疫组织化学方法检测了436例乳腺癌组织切片中组织蛋白酶D的表达。组织蛋白酶D阳性肿瘤细胞比例与肿瘤大小及激素受体状态无关，仅与增殖活性弱相关，可作为H-3-胸腺嘧啶核苷标记指数。相反，在结节阳性的肿瘤中，阳性细胞的比例高于结节阴性的肿瘤(p=0.05)。对100例单个肿瘤的免疫组织化学和免疫放射学结果进行了配对比较，发现两者之间存在显著的相关性，但相关系数为0.46。两种检测结果在ER-肿瘤中的一致性高于ER+肿瘤，后者有时仅限于巨噬细胞和正常上皮细胞。原位评估的主要优点是特别适用于肿瘤细胞的检测，并允许同时确定不同的生物学方面，以更全面地了解乳腺癌生物学。
The expression of cathepsin D was evaluated by immunohistochemistry on histologic sections from formalin-fixed samples in a series of 436 primary breast cancers. The fraction of cathepsin D-positive tumor cells was not related to tumor size or hormone receptor status, and only weakly related to proliferative activity, evaluated as the H-3-thymidine labeling index. Conversely, a higher fraction of positive cells was observed in node-positive than in node-negative tumors (p = 0.05). A matched comparison between immunohistochemical and immunoradiometric results on individual tumors was carried out on 100 cases and showed a significant association but with a correlation coefficient of 0.46. The agreement of results from the two assays was higher in ER- than in ER+ tumors, which sometimes showed an immunostaining limited to macrophages and normal epithelial cells. In situ evaluation has the main advantage of being specifically applicable to detection in tumor cells and allows the simultaneous determination of different biologic aspects for a more complete understanding of breast cancer biology.