In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope.
In vitro investigation of efficient photodynamic therapy using a nonviral vector; hemagglutinating virus of Japan envelope.
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DOI：
10.1117/1.jbo.17.7.078002
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发表时间：
2012-07
影响因子：
3.5
通讯作者：
M. Sakai;N. Fujimoto;K. Ishii;Hiroyuki Nakamura;Y. Kaneda;K. Awazu
中科院分区：
文献类型：
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作者：
M. Sakai;N. Fujimoto;K. Ishii;Hiroyuki Nakamura;Y. Kaneda;K. Awazu
光动力疗法（PDT）是一种光化学疗法，被批准用于癌症治疗。PDT已在早期肺癌和食管癌中显示出有效性。光敏剂在癌细胞中的积累对于增强PDT的治疗益处是必要的;然而，光敏剂具有低摄取效率。为了克服这种限制，需要药物递送系统，例如日本包膜血凝病毒（HVJ-E）载体。在这项研究中，PDT和HVJ-E的组合进行了研究，以提高PDT的疗效。评价的光敏剂包括5-氨基乙酰丙酸（5-ALA）、原卟啉IX（PPIX）和HVJ-PPIX。随着HVJ-E的加入，光敏剂的摄取增加了20倍。通过添加HVJ-E载体增强了常规5-ALA的细胞毒性。总之，HVJ-E载体提高了光敏剂的摄取和PDT效应。
Photodynamic therapy (PDT) is a photochemical modality approved for cancer treatment. PDT has demonstrated efficacy in early stage lung cancer and esophageal cancer. The accumulation of photosensitizers in cancer cells is necessary to enhance the therapeutic benefits of PDT; however, photosensitizers have low uptake efficiency. To overcome this limitation, a drug delivery system, such as the hemagglutinating virus of Japan envelope (HVJ-E) vector, is required. In this study, the combination of PDT and HVJ-E was investigated for enhancing the efficacy of PDT. The photosensitizers that were evaluated included 5-aminolaevulinic acid (5-ALA), protoporphyrin IX (PPIX), and HVJ-PPIX. The uptake of the photosensitizers as increased twenty-fold with the addition of HVJ-E. The cytotoxicity of conventional 5-ALA was enhanced by the addition of HVJ-E vector. In conclusion, HVJ-E vector improved the uptake of photosensitizers and the PDT effect.