In vitro multichannel single-unit recordings of action potentials from the mouse sciatic nerve
In vitro multichannel single-unit recordings of action potentials from the mouse sciatic nerve
复制标题
DOI：
10.1088/2057-1976/aa7efa
复制
发表时间：
2017-08-01
影响因子：
1.4
通讯作者：
Feng, B.
中科院分区：
文献类型：
--
作者：
Chen, L.;Ilham, S. J.;Feng, B.
与外周神经连接的电极阵列对于靶向外周器官以缓解症状的神经调节装置是必不可少的。调制（即，单个单元记录和刺激）单个外周神经轴突仍然是一个技术挑战。在这里，我们报告了一个体外设置，允许同时从多个小鼠坐骨神经轴突的单单位记录。收集坐骨神经（类似于30 mm）并转移到组织室中，将类似于5 mm的远端拉入填充有石蜡油的相邻记录室中。使用定制的多丝电极阵列与分裂的细神经丝连接。电刺激诱发186个轴突的单位动作电位，其中传导速度（CV）大于1 ms（-1）的A型占49.5%，C型占50.5%（CV < 1 ms（-1））。单单位记录对A型或C型轴突没有明显的偏向，在60分钟内是稳健的和可重复的，因此是评估靶向外周神经的不同神经调节策略的理想机会。例如，使用该装置评估动作电位传输的超声调制，表明超声刺激后神经传导速度增加。该设置也可用于客观评估与外周神经连接的下一代电极阵列的设计。
Electrode arrays interfacing with peripheral nerves are essential for neuromodulation devices targeting peripheral organs to relieve symptoms. To modulate (i.e., single-unit recording and stimulating) individual peripheral nerve axons remains a technical challenge. Here, we report an in vitro setup to allow simultaneous single-unit recordings from multiple mouse sciatic nerve axons. The sciatic nerve (similar to 30 mm) was harvested and transferred to a tissue chamber, the similar to 5 mm distal end pulled into an adjacent recording chamber filled with paraffin oil. A custom-built multi-wire electrode array was used to interface with split fine nerve filaments. Single-unit action potentials were evoked by electrical stimulation and recorded from 186 axons, of which 49.5% were classed A-type with conduction velocities (CV) greater than 1 ms(-1) and 50.5% were C-type (CV < 1 ms(-1)). The single-unit recordings had no apparent bias towards A-or C-type axons, were robust and repeatable for over 60 min, and thus an ideal opportunity to assess different neuromodulation strategies targeting peripheral nerves. For instance, ultrasonic modulation of action potential transmission was assessed using the setup, indicating increased nerve conduction velocity following ultrasound stimulus. This setup can also be used to objectively assess the design of next-generation electrode arrays interfacing with peripheral nerves.