Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor
Cloning, expression analysis, and regulatory mechanisms of bovine chemerin and chemerin receptor
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DOI：
10.1016/j.domaniend.2010.02.007
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发表时间：
2010-08-01
影响因子：
2.1
通讯作者：
Katoh, K.
中科院分区：
文献类型：
--
作者：
Song, S. -H.;Fukui, K.;Katoh, K.
最近，我们报道了一种新的脂肪因子chemerin，在小鼠脂肪组织中高表达，在脂肪细胞分化过程中上调，并通过其自身的受体调节脂肪形成。本研究的目的是克隆chemerin及其受体从脂肪组织的日本黑牛，并调查这些基因的表达在16个不同的组织。我们比较了从皮下脂肪组织制备的脂肪细胞和基质血管（S-V）细胞（非脂肪细胞）之间chemerin及其受体的基因表达。此外，我们还研究了趋化素及其受体在牛分化脂肪细胞中的mRNA表达水平。测定了牛趋化素及其受体的DNA序列，发现它们与人、小鼠和猪的趋化素及其受体的DNA序列高度同源。牛chemerin及其受体的全长cDNA预测的氨基酸序列也类似于人类，小鼠和猪，这表明这些基因具有相似的功能。牛chemerin mRNA在脂肪和肝组织中高度表达，chemerin受体转录本在脂肪、肌肉、肝和脑组织中广泛表达。牛chemerin mRNA在脂肪细胞中的表达高于从脂肪组织制备的S-V细胞。在脂肪细胞分化过程中，chemerin及其受体的转录水平上调。用肿瘤坏死因子（TNF）-α（10 ng/mL）处理牛分化的脂肪细胞可增加chemerin及其受体的mRNA表达。这些结果表明，chemerin，一个新的脂肪细胞因子在牛脂肪组织的脂肪细胞中高度表达，是TNF-α上调基因，在脂肪形成中发挥作用。(C)2010年爱思唯尔公司All rights reserved.
Recently, we reported that chemerin, a new adipokine, is highly expressed in the adipose tissue, up-regulated during adipocyte differentiation, and regulates adipogenesis via its own receptor in mice. The objectives of this study were to clone chemerin and its receptor from the adipose tissues of Japanese Black cattle and to investigate the expression of these genes in 16 different tissues. We compared the gene expression of chemerin and its receptor between adipocytes and stromal-vascular (S-V) cells (non-adipocytes) prepared from subcutaneous adipose tissue. In addition, we investigated the mRNA expression levels of chemerin and its receptor in bovine differentiated adipocytes. The DNA sequences of bovine chemerin and its receptor were determined, and they were found to be highly homologous to those of humans, mice, and pigs. The amino acid sequences predicted for the full-length cDNA of bovine chemerin and its receptor were also similar to those of humans, mice, and pigs, suggesting that these genes have similar functions. Bovine chemerin mRNA was highly expressed in the adipose and liver tissues, and the transcripts of chemerin receptor were widely expressed in several tissues including adipose, muscle, liver, and brain tissues. The expression of bovine chemerin mRNA was higher in adipocytes than in S-V cells prepared from adipose tissue. The transcripts of chemerin and its receptor were up-regulated during adipocyte differentiation. Treatment with tumor necrosis factor (TNF)-alpha (10 ng/mL) in bovine differentiated adipocytes increased the mRNA expression of chemerin and its receptor. These results indicate that chemerin, a new adipokine highly expressed in the adipocytes of bovine adipose tissue, is the TNF-alpha-up-regulated gene with a role in adipogenesis. (C) 2010 Elsevier Inc. All rights reserved.