Magnetic resonance lactate and lipid signals in rat brain after middle cerebral artery occlusion model
Magnetic resonance lactate and lipid signals in rat brain after middle cerebral artery occlusion model
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DOI：
10.1016/j.brainres.2006.11.075
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发表时间：
2007-02-23
期刊：
影响因子：
2.9
通讯作者：
Kocsis, Jeffery D.
中科院分区：
文献类型：
--
作者：
Harada, Kuniaki;Honmou, Osamu;Kocsis, Jeffery D.
质子磁共振波谱(1-HMRS)显示了急性脑梗塞时代谢产物的变化。虽然乳酸和N-乙酰天冬氨酸的急剧变化已被报道为人类和实验动物脑缺血损伤的有用指标，但在缺血15天后，短回声时间序列也可以检测到脂质信号。这项研究的目的是寻找一种新的技术来分离缺血脑中的乳酸信号和脂质信号。首先，利用MRS对球形体模的脂类和乳酸成分进行了体外研究，并建立了体外分离这些成分的参数。然后，对大脑中动脉阻塞大鼠的脑组织进行MR测量。所有磁共振测量均使用7-T(300 MHz)、18.3厘米口径的超导磁体(牛津磁铁技术公司)与Unity INOVA成像系统(瓦里安技术公司)接口进行。T-2加权图像来自1.0 mm厚的冠状面，使用3 cm视野。众所周知，脂质具有较短的T-2松弛时间，而乳酸具有较长的T-2松弛时间。这些独特的磁性特征使我们能够将乳酸信号与脂肪信号分开。因此，对回声时间的调整对于分析急性脑梗塞患者的代谢产物是至关重要的，这对临床和实验室都有一定的参考价值。(C)2006爱思唯尔B.V.保留所有权利。
Proton magnetic resonance spectroscopy (1-H MRS) has revealed changes of metabolites in acute cerebral infarction. Although the drastic changes of lactate and N-acetyl-aspartate have been reported to be useful indicators of the ischemic damage in both humans and experimental animals, lipid signals are also detected by the short echo time sequence 15 days after ischemia. The objective of this study was to find a novel technique to isolate lactate signals from lipid signals in the ischemic brain. First, MRS was used to study the lipid and lactate components of a spherical phantom in vitro, and parameters were established to separate these components in vitro. Then, MR measurements were obtained from the brains of middle cerebral artery occlusion rats. All MR measurements were performed using a 7-T (300 MHz), 18.3-cm-bore superconducting magnet (Oxford Magnet Technologies) interfaced to a Unity INOVA Imaging System (Varian Technologies). T-2-weighted images were obtained from a 1.0-mm-thick coronal section using a 3-cm field of view. It is well known that lipid has a shorter and lactate a longer T-2 relaxation time. These distinct magnetic characteristics allowed us to separate the lactate signal from the lipid signal. Thus, adjustment of the echo time is essential to analyze the metabolites in acute cerebral infarction, which maybe useful in both the clinic and laboratory. (c) 2006 Elsevier B.V. All rights reserved.