Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
Divalent cations can control a switch-like behavior in heterotypic and homotypic RNA coacervates
复制标题
DOI：
10.1038/s41598-019-48457-x
复制
发表时间：
2019-08-21
影响因子：
4.6
通讯作者：
Banerjee, Priya R.
中科院分区：
文献类型：
--
作者：
Onuchic, Paulo L.;Minn, Anthony N.;Banerjee, Priya R.
RNA-蛋白质复合物的液-液相分离（LLPS）在无膜细胞器（MLO）的细胞功能中起着重要作用。MLO对细胞条件的变化敏感，例如细胞质离子浓度的波动。为了研究这些变化对MLO的影响，我们研究了二价阳离子对RNA凝聚体的物理和化学性质的影响。使用一个模型系统，包括一个富含精氨酸的肽和RNA，我们预测和观察到，在信号阳离子的变化发挥相互作用依赖的影响RNA LLPS。改变离子环境对异型肽-RNA和同型RNA LLPS的倾向具有相反的影响，这导致凝聚层类型之间的切换。此外，二价离子的变化不断调整异型和同型液滴的微环境和流体性质。我们的研究结果可能提供一个一般的机制，在细胞环境中调节RNA凝聚体的生化环境。
Liquid-liquid phase separation (LLPS) of RNA-protein complexes plays a major role in the cellular function of membraneless organelles (MLOs). MLOs are sensitive to changes in cellular conditions, such as fluctuations in cytoplasmic ion concentrations. To investigate the effect of these changes on MLOs, we studied the influence of divalent cations on the physical and chemical properties of RNA coacervates. Using a model system comprised of an arginine-rich peptide and RNA, we predicted and observed that variations in signaling cations exert interaction-dependent effects on RNA LLPS. Changing the ionic environment has opposing effects on the propensity for heterotypic peptide-RNA and homotypic RNA LLPS, which results in a switch between coacervate types. Furthermore, divalent ion variations continuously tune the microenvironments and fluid properties of heterotypic and homotypic droplets. Our results may provide a general mechanism for modulating the biochemical environment of RNA coacervates in a cellular context.