Effects of different carbon sources on the efficiency of sulfur-oxidizing denitrifying microorganisms
Effects of different carbon sources on the efficiency of sulfur-oxidizing denitrifying microorganisms
复制标题
不同碳源对硫氧化反硝化微生物效率的影响
DOI：
10.1016/j.envres.2021.111946
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发表时间：
2021-09-13
影响因子：
8.3
通讯作者：
Huang, Cong
中科院分区：
文献类型：
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作者：
Gao, Shuang;Li, Zhiling;Huang, Cong
以葡萄糖、乙醇和乙酸盐为碳源，比较不同碳源对硫氧化微生物的影响。在相同的化学需氧量Cr（CODCr）、硝酸盐和硫化物浓度下，以葡萄糖为碳源的静态实验和连续流反应器对硝酸盐和总有机碳的去除率以及元素硫的产率均低于以乙醇和乙酸为碳源的实验.以葡萄糖为碳源的核心硫氧化降解菌为固氮菌属、地碱杆菌属和芒果弯曲菌属;以乙醇为碳源的核心硫氧化降解菌为Arcobacter、假单胞菌属和Thauera;以乙酸盐为碳源的核心硫氧化降解菌为假单胞菌属和固氮菌属。通过功能基因检测解释了利用不同碳源的微生物的代谢活性。指示微生物异养代谢的功能基因gltA在3个反应器中波动较小，而硫氧化基因Sqr在以乙酸为碳源的反应器中波动较大。研究结果表明，乙酸盐是一种更适合于脱硫-脱硫系统的碳源。
This study aims to compare the effects of different carbon sources on sulfur-oxidizing denitrifying microorganisms by using glucose, ethanol, and acetate as carbon sources. Under the same chemical oxygen demand Cr (CODCr), nitrate, and sulfide concentrations, the removal rate of nitrate and total organic carbon, and the yield of elemental sulfur in a static experiment and a continuous flow reactor with glucose as the carbon source were lower than those with ethanol and acetic acid as the carbon source. The core sulfur-oxidizing denitrifying bacteria that use glucose as the carbon source were Azoarcus, Geoalkalibacter, and Mangroviflexus; those that use ethanol as the carbon source were Arcobacter, Pseudomonas, and Thauera; those that use acetate as the carbon source were Pseudomonas and Azoarcus. The metabolic activity of microorganisms that use different carbon sources was explained by functional gene detection. The fluctuation of gltA, a functional gene indicating heterotrophic metabolism of microorganisms, was small in three reactors, but that of the sulfur oxidation gene, Sqr, in the reactor with acetic acid as the carbon source was larger. Our results suggest that acetate is a more suitable carbon source for denitrification-desulfurization systems.