Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine.
Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine.
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DOI：
10.1016/j.vaccine.2015.09.055
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发表时间：
2015-10
期刊：
影响因子：
5.5
通讯作者：
Haixue Zheng;Kaiqi Lian;Fan Yang;Ye Jin;Zixiang Zhu;Jian-hong Guo;W. Cao;Huanan Liu;Jijun He;Keshan Zhang;Dan Li;Xiang-tao Liu
中科院分区：
文献类型：
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作者：
Haixue Zheng;Kaiqi Lian;Fan Yang;Ye Jin;Zixiang Zhu;Jian-hong Guo;W. Cao;Huanan Liu;Jijun He;Keshan Zhang;Dan Li;Xiang-tao Liu
口蹄疫（FMD）是一种高度传染性的水泡性疾病，影响世界范围内的家养和野生偶蹄动物。近年来，东南亚国家、中国、俄罗斯联邦、蒙古、哈萨克斯坦和韩国等国相继发生了甲型口蹄疫疫情。来自中国广东省（2013年）的FMD病毒（A/GDMM/CHA/2013）是造成最新流行病的代表，并且与2009年来自中国武汉的流行毒株A/WH/CHA/09在VP 1蛋白中具有低氨基酸同一性（93.9%）。两株分离株均属于ASIA拓扑型的Sea-97基因型。因此，应用具有交叉保护效力的新疫苗株对于控制两种所述大流行毒株的传播至关重要。本实验室将口蹄疫流行株A/WH/CHA/09的P1基因替换疫苗株O/CHA/99中的P1基因，构建了重组口蹄疫病毒（rA/P1-FMDV）嵌合株，经体外培养试验证明，重组口蹄疫病毒（rA/P1-FMDV）灭活疫苗具有良好的生长特性，可对口蹄疫流行株A/WH/CHA/09提供免疫保护。然而，目前尚不清楚该疫苗是否能有效预防新的大流行毒株（A/GDMM/CHA/2013）。在此，进行了疫苗匹配和猪50%保护剂量（PD 50）试验，以评估疫苗效力。全剂量疫苗免疫猪血清与A/WH/CHA/09和A/GDMM/CHA/2013分离株交叉反应，平均1值分别为0.94 ± 0.12和0.68 ± 0.06（r1≥ 0.3），表明rA/P1-FMDV疫苗对两株分离株可能具有良好的交叉保护作用。用两株大流行分离株A/WH/CHA/09和A/GDMM/CHA/2013株攻毒，疫苗每剂（2.8 μg）分别获得12.51和10.05 PD 50。结果表明，rA/P1-FMDV灭活疫苗对A/WH/CHA/09和A/GDMM/CHA/2013大流行株均有免疫保护作用。
Foot-and-mouth disease (FMD) is a highly contagious vesicular disease that affects domestic and wild cloven-hoofed animals worldwide. Recently, a series of outbreaks of type A FMDV occurred in Southeast Asian countries, China, the Russia Federation, Mongolia, Kazakhstan and South Korea. The FMD virus (A/GDMM/CHA/2013) from China's Guangdong province (2013) is representative of those responsible for the latest epidemic, and has low amino acid identity (93.9%) in VP1 protein with the epidemic strain A/WH/CHA/09 from Wuhan, China in 2009. Both of isolates belong to the Sea-97 genotype of ASIA topotype. Therefore, the application of a new vaccine strain with cross-protective efficacy is of fundamental importance to control the spread of the two described pandemic strains. A chimeric strain rA/P1-FMDV constructed by our lab previously through replacing the P1 gene in the vaccine strain O/CHA/99 with that from the epidemic stain A/WH/CHA/09, has been demonstrated to exhibit good growth characteristics in culture, and the rA/P1-FMDV inactivated vaccine can provide protection against epidemic strain A/WH/CHA/09 in cattle. However, it is still unclear whether the vaccine produces efficient protection against the new pandemic strain (A/GDMM/CHA/2013). Here, vaccine matching and pig 50% protective dose (PD50) tests were performed to assess the vaccine potency. The vaccine matching test showed cross-reactivity of sera from full dose vaccine vaccinated pigs with A/WH/CHA/09 and A/GDMM/CHA/2013 isolates, with averager1values of 0.94 ± 0.12 and 0.68 ± 0.06 (r1≥ 0.3), which indicates that the rA/P1-FMDV vaccine is likely to confer good cross-protection against the two isolates. When challenged with two pandemic isolates A/WH/CHA/09 and A/GDMM/CHA/2013 strain, the vaccine achieved 12.51 PD50and 10.05 PD50per dose (2.8 μg), respectively. The results indicated that the rA/P1-FMDV inactivated vaccine could protect pigs against both A/WH/CHA/09 and A/GDMM/CHA/2013 pandemic isolates.