The fate of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) from livestock wastewater (dominated by quinolone antibiotics) treated by microbial fuel cell (MFC)
The fate of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) from livestock wastewater (dominated by quinolone antibiotics) treated by microbial fuel cell (MFC)
复制标题
微生物燃料电池（MFC）处理的牲畜废水（以喹诺酮类抗生素为主）中抗生素抗性基因（ARG）和移动遗传元件（MGE）的命运
DOI：
10.1016/j.ecoenv.2021.112267
复制
发表时间：
2021-04-28
影响因子：
6.8
通讯作者：
Wei, Zhaolan
中科院分区：
文献类型：
--
作者：
Chen, Jia;Wang, Tingting;Wei, Zhaolan
采用高通量定量(HTqPCR)技术对MFC处理后畜禽废水(以喹诺酮类抗生素为主)中抗生素耐药基因和可移动基因的去除特性进行了评价。结果表明，在畜禽废水中检出144个ARGs和8个Megs。经MFC处理后，AGRs的数量整体减少，大环内酯类-林可胺链球菌B组(MLSB)和氨基糖苷类药物的相对丰度分别降低了62.7%和92.9%。MGES减少57.3%，多药基因减少90%。经MFC处理后，原污水中四环素的绝对丰度从5.8×105拷贝L-1下降到5.1×103拷贝L-1，下降了两个数量级。然而，MFC在去除万古霉素和β-内酰胺酶基因方面效率较低。氯霉素耐药基因略有增加。Illumina测序表明，MFC中以合养细菌和协生细菌为主。脱硫弧菌对高浓度盐酸莫西沙星耐药。当L-1浓度为5 mg时，MFC对盐酸莫西沙星的去除效率为86.55%。最大功率密度为109.3 mV.cm-3，库仑效率为41.97%。随着抗生素浓度的增加，污水处理效率和电学性能受到抑制。本研究表明，未经处理的畜禽废水存在较大的基因水平转移风险。尽管MFC对高浓度喹诺酮类废水的处理能力有限，但它是减少ARGs和水平基因转移风险的有效方法。
The removal characteristics of antibiotic resistance genes and mobile genetic elements from livestock wastewater (dominated by quinolone antibiotics) treated with MFC were evaluated by High-throughput quantitative (HTqPCR). The results showed that 144 ARGs and 8 MEGs were detected in the livestock wastewater. After MFC treatment, the number of AGRs decreased as a whole, and the relative abundance of macrolide-lincosamidestreptogramin group B (MLSB) and aminoglycosider decreased by 62.7% and 92.9%, respectively. MGEs decreased by 57.3% and multidrug genes decreased by 90%. After MFC treatment, the absolute abundance of tetracycline in raw sewage decreased by two orders of magnitude from 5.8 x 105 copies L-1 to 5.1.x 103 copies L-1. However, MFC was less efficient in the removal of vancomycin and beta-lactamase genes. It was also found that chloramphenicol resistance genes slightly increased. Illumina sequencing showed that Syntrophobacterales and Synergistales were predominant in MFCs. Desulfovibrio was resistant to high concentration of moxifloxacin hydrochloride. The removal efficiency of MFC for moxifloxacin hydrochloride at a concentration of 5 mg L-1 was 86.55%. The maximum power density and coulomb efficiency were 109.3 mV.cm- 3 and 41.97%, respectively. With the increase of antibiotic concentration, the sewage treatment efficiency and electrical performance were inhibited. This study shows that untreated livestock wastewater had a great risk of gene horizontal transfer. Although MFC had limited treatment capacity for high-concentration quinolone wastewater, it is an effective method to reduce ARGs and the risk of horizontal gene transfer.