zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly.
zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly.
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Zeta-cop是涂层囊泡组件所必需的。
DOI：
10.1083/jcb.123.6.1727
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发表时间：
1993-12
影响因子：
7.8
通讯作者：
Rothman, J E
中科院分区：
文献类型：
--
作者：
Kuge, O;Hara-Kuge, S;Orci, L;Ravazzola, M;Amherdt, M;Tanigawa, G;Wieland, F T;Rothman, J E
cDNA编码的20 kD亚基的外套，zeta-COP，预测的177个氨基酸残基的蛋白质，类似于在序列中的AP 17和AP 19，亚基的网格蛋白适配器复合物。针对zeta-COP的多克隆抗体阻断了coatomer与高尔基体膜的结合，并阻止了COP包被的囊泡在高尔基体池上的组装。与其他外被体亚基（β-、β '-、γ-和β- COP）不同，zeta-COP存在于外被体结合池和游离池两者中。
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon- COP), zeta-COP exists in both coatomer bound and free pools.