An investigation of the effectiveness of certain antioxidants in preserving the motility of reactivated bull sperm models.
An investigation of the effectiveness of certain antioxidants in preserving the motility of reactivated bull sperm models.
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研究某些抗氧化剂在保持重新激活的公牛精子模型活力方面的有效性。
DOI：
10.1095/biolreprod38.1.114
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发表时间：
1988
影响因子：
3.6
通讯作者：
Giblin,FJ
中科院分区：
文献类型：
--
作者：
Lindemann,CB;O'Brien,JA;Giblin,FJ
用1 mM Mg-腺苷5 ′-三磷酸重新激活冻融破坏的公牛精子。抗氧化剂超氧化物歧化酶，过氧化氢酶，二硫苏糖醇，还原型谷胱甘肽（GSH）的能力进行了测试，以延长活力的重新激活的精子。GSH既可以单独使用，也可以作为还原系统的一部分，使三肽保持还原形式。发现三种测试试剂增加精子制备物的运动持续时间;这些试剂是还原型谷胱甘肽、二硫苏糖醇和超氧化物歧化酶。谷氨酸是最有效的保护剂，产生的再活化制剂的半衰期为2.5小时的运动衰减。虽然二硫苏糖醇（DTT）被广泛用作抗氧化剂，但我们发现DTT的有效性明显低于谷胱甘肽（半衰期为1.5小时）。尽管谷胱甘肽的有效性，在保持活力，我们已经发现，通过直接测定，成熟的公牛精子不包含检测量的这种常见的生物抗氧化剂。我们的研究结果支持了这一假设，即氧化损伤有助于恢复活力的精子活力的损失，并表明，氧化可能是活精子活力损失的一个因素。
Bull sperm that had been disrupted by freezing and thawing were reactivated with 1 mM Mg-adenosine 5′-triphosphate. The antioxidants superoxide dismutase, catalase, dithiothreitol, and reduced glutathione (GSH) were tested for their ability to prolong the motility of the reactivated sperm. GSH was employed both by itself and as part of a reducing system that maintained the tripeptide in the reduced form. Three of the test agents were found to increase the duration of motility in the sperm preparations; these were reduced gluta¬thione, dithiothreitol, and superoxide dismutase. Glutathione was the most effective protective agent, yielding reactivated preparations with a half-life for the decay of motility of 2.5 h. While dithiothreitol (DTT) is widely employed as an antioxidant, we found that DTT is measurably less effective than glutathione (half-life of 1.5 h). In spite of glutathione’s effectiveness in preserving motility, we have found, by direct assay, that mature bull sperm do not contain detectable amounts of this common biological antioxidant. Our results support the hypothesis that oxidative damage contributes to the loss of motility in reactivated sperm and suggest that oxidation could be a factor in motility loss in living sperm.