Alterations of gene expression in human neutrophils induced by smoking cessation
Alterations of gene expression in human neutrophils induced by smoking cessation
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DOI：
10.1111/j.1600-051x.2004.00612.x
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发表时间：
2004-12-01
影响因子：
6.7
通讯作者：
Yoshie, H
中科院分区：
文献类型：
--
作者：
Morozumi, T;Kubota, T;Yoshie, H
目的：本研究的目的是探讨戒烟对外周中性粒细胞炎症细胞因子、趋化因子、生长因子和基质金属蛋白酶（MMP）mRNA表达水平的影响。材料与方法：招募16名无牙周和全身疾病临床症状的男性吸烟者（年龄22-39 [25.3+/-4.0]岁）。该实验在戒烟前（基线）以及戒烟后 1、4 和 8 周进行。通过呼出一氧化碳（CO）浓度和血清可替宁浓度来验证吸烟和戒烟状况。从每位受试者的外周血中分离出中性粒细胞，然后用 N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）刺激细胞。通过半定量逆转录聚合酶链反应分析白细胞介素（IL）-1β、IL-8、肿瘤坏死因子（TNF）-α、血管内皮生长因子（VEGF）和MMP-8的mRNA表达水平。对年龄 23-27 (24.4+/-1.2) 岁的 11 名不吸烟对照者（4 名女性和 7 名男性）进行了相同的实验。结果：16 名吸烟者中有 11 人成功完成戒烟 8 周。戒烟后 1 天，CO 浓度在统计上显着低于基线 (p
Objective: The purpose of the present study was to investigate the effect of smoking cessation on the peripheral neutrophil mRNA expression levels for inflammatory cytokines, chemokine, growth factor and matrix metalloproteinase (MMP).Material and Methods: Sixteen male smokers (aged 22-39 [25.3+/-4.0] years), with no clinical signs of periodontal and systemic diseases, were recruited. The experiment was performed before (baseline) and at 1, 4 and 8 weeks after smoking cessation. The status of smoking and smoking cessation was verified by exhaled carbon monoxide (CO) concentration and serum cotinine concentration. Neutrophils were isolated from each subjects' peripheral blood, then the cell was stimulated with N-formyl-methionyl-leucyl-phenylalanine (FMLP). The mRNA expression levels for interleukin (IL)-1beta, IL-8, tumor necrosis factor (TNF)-alpha, vascular endothelial growth factor (VEGF) and MMP-8 were analyzed by semiquantitative reverse transcription-polymerase chain reactions. The same experiment was performed on 11 non-smoking controls (four female and seven male), aged 23-27 (24.4+/-1.2) years.Results: Eleven of 16 smokers successfully completed smoking cessation for 8 weeks. At 1 day after smoking cessation, there was a statistically significantly lower CO concentration than at baseline (p