A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)
A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis)
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DOI：
10.3724/sp.j.1035.2008.00019
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Wang Ding
中科院分区：
文献类型：
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作者：
Zheng Jin-Song;Liao Xiao-Lin;Wang Ding
为开发濒危物种长江江豚的微卫星标记，采用改进的FASSCO(快速分离包含重复序列的AFLP)方法分离了72条江豚微卫星DNA序列。根据Weber的分类规则，将这些序列分为三类：完全重复序列(占总数的58.3%)、不完全重复序列(22.2%)和复合重复序列(19.5%)。设计合成了30对聚合酶链式反应(PCR)打印子，随机抽取12头长江江豚作为样本进行筛选。结果表明，其中14个群体表现出2~13个等位基因，平均为5.86个等位基因/座位。它们的平均观察杂合度和期望杂合度分别为0.560和0.709。这些是长江江豚的第一批特异微卫星标记，在后续的保护遗传学研究中将发挥重要作用。
To the aim of developing specific microsatellite markers for the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis 72 microsatellite DNA sequences were isolated using an improved FIASCO (Fast Isolation by AFLP of Sequences Containing Repeats) protocol. According to Weber's classification rules, these sequences were divided into three categories: perfect repeat sequences (58.3% of total), imperfect repeat sequences (22.2%), and compound repeat sequences (19.5%). Thirty PCR printer pairs were designed and synthesized, and then screened by a sample panel of 12 random selected Yangtze finless porpoises. As a result, 14 of them were proved to be polymorphic exhibiting 2 to 13 alleles (mean 5.86 alleles/locus). Their average observed heterozygosity and expected heterozygosity were 0.560 and 0.709 respectively. These are the first batch of specific microsatellite markers for the Yangtze finless porpoise, and they should play great role in succedent conservation genetics studies.