A conserved RING finger protein required for histone H2B monoubiquitination and cell size control
A conserved RING finger protein required for histone H2B monoubiquitination and cell size control
复制标题
DOI：
10.1016/s1097-2765(02)00826-2
复制
发表时间：
2003-01-01
期刊：
影响因子：
16
通讯作者：
Madhani, HD
中科院分区：
文献类型：
--
作者：
Hwang, WW;Venkatasubrahmanyam, S;Madhani, HD
组蛋白H2B的单泛素化是赖氨酸4 （K4）上组蛋白H3甲基化所必需的，这是一种与活性染色质相关的修饰。同源的泛素连接酶的身份是未知的。我们发现Bre1是一个进化上保守的无名指蛋白，在体内是H2B泛素化和h3k4甲基化所必需的。Bre1的RING结构域对于这两种修饰都是必不可少的，Lge1（大1）也是如此，Lge1是一种与Bre1结合的细胞大小控制所需的蛋白质。在缺乏常染色质相关组蛋白变体H2A的细胞中。Z、BRE1、RAD6和LGE1都是细胞生存所必需的，在活性染色质形成过程中支持H2B泛素化和H2A替代的冗余功能。值得注意的是，突变体分析表明Bre1/ lge1依赖性H2B单泛素化在控制细胞大小方面具有功能。
Monoubiquitination of histone H2B is required for methylation of histone H3 on lysine 4 (K4), a modification associated with active chromatin. The identity of the cognate ubiquitin ligase is unknown. We identify Bre1 as an evolutionarily conserved RING finger protein required in vivo for both H2B ubiquitination and H3 K4 methylation. The RING domain of Bre1 is essential for both of these modifications as is Lge1 (Large 1), a protein required for cell size control that copurifies with Bre1. In cells lacking the euchromatin-associated histone variant H2A.Z, BRE1, RAD6, and LGE1 are each essential for cell viability, supporting redundant functions for H2B ubiquitination and H2A substitution in the formation of active chromatin. Notably, analysis of mutants demonstrates a function for Bre1/Lge1-dependent H2B monoubiquitination in the control of cell size.