Flow cytometric DNA analysis for the determination of malignant potential in adrenal and extra-adrenal pheochromocytomas or paragangliomas.
Flow cytometric DNA analysis for the determination of malignant potential in adrenal and extra-adrenal pheochromocytomas or paragangliomas.
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发表时间：
1993-11
影响因子：
4.6
通讯作者：
L. Pang;K. Tsao
中科院分区：
文献类型：
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作者：
L. Pang;K. Tsao
用流式细胞术对53例肾上腺、13例颈动脉体部、14例腹膜后、2例胸腔内、2例膀胱和1例马尾嗜铬细胞瘤(副神经节瘤)的细胞核进行了DNA倍体研究。一种特殊的技术用于将石蜡包埋的组织制备到单个相关核中，另一种特殊的方法用于碘化丙啶染色。20个正常肾上腺也作为对照进行分析。6例肾上腺髓质肿瘤由于细胞碎片过多或其他技术故障而产生无法解释的直方图(变异系数为7%)。对照组肾上腺均为二倍体。临床上72个肿瘤是良性的，7个是恶性的，这是区域或远处转移和/或广泛的局部侵犯的证据。在79个肿瘤中，56个(71%)显示正常的DNA二倍体模式，临床病程为良性。其余23例(包括7例恶性肿瘤)DNA直方图异常，包括四倍体和非整倍体。DNA四倍体组和DNA异倍体组的恶性肿瘤发生率明显高于DNA正常组，DNA直方图异常的两组与DNA正常组相比差异有统计学意义。在这些结果的基础上，非二倍体肿瘤被认为比二倍体肿瘤更容易发生侵袭行为，因此应该仔细监测。
DNA ploidy studies were performed by flow cytometry on extracted nuclei from 53 adrenal, 13 carotid body, 14 retroperitoneal, two intrathoracic, two urinary bladder, and one cauda equina pheochromocytomas (paragangliomas). A specific technique was used for preparation of paraffin-embedded tissue into single associated nuclei, and another specific method was used for propidium iodide staining. Twenty normal adrenal glands were also analyzed as controls. Six tumors in the adrenal medulla yielded uninterpretable histograms (coefficient of variation > 7%) because of excessive cell debris or other technical failure. All control adrenal glands were diploid. Seventy-two tumors were clinically benign and seven were malignant as evidenced by regional or distant metastases and/or extensive local invasion. Fifty-six (71%) of 79 tumors disclosed a normal DNA diploidy pattern with a benign clinical course. Abnormal DNA histograms including tetraploidy and aneuploidy patterns were observed in the remaining 23 cases, including the seven malignant tumors. Significantly more malignant tumors occurred in the DNA tetraploid group and the DNA aneuploid group than in the normal DNA group; considered together, the two groups with abnormal DNA histograms differed significantly from the normal DNA group. On the basis of these results, nondiploid tumors are considered to be more prone to aggressive behavior than diploid tumors and, therefore, should be carefully monitored.