Biohydroxylation reactions catalyzed by enzymes and whole-cell systems
Biohydroxylation reactions catalyzed by enzymes and whole-cell systems
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DOI：
10.1006/bioo.1999.1135
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发表时间：
1999-04-01
影响因子：
5.1
通讯作者：
Staines, A
中科院分区：
文献类型：
--
作者：
Flitsch, SL;Aitken, SJ;Staines, A
使用含有芳香族侧链的许多环状底物（3-24）的生物羟基化来比较使用微生物酶和全细胞生物催化剂的羟基化的底物特异性和选择性。在一般情况下，反应的区域选择性是显着相似的不同催化剂之间，很少的芳香族或苄基，但观察到显着的脂肪族羟基化。然而，更详细的调查分离的产品显示互补底物特异性，官能团的相容性，和区域选择性的羟基化。底物特异性和区域选择性可以通过对芳族侧链的性质的微小改变来进一步调节，芳族侧链似乎在底物识别中起重要作用，（C）1999 Academic Press。
The biohydroxylation of a number of cyclic substrates (3-24) containing aromatic side chains was used to compare substrate specificity and selectivity of hydroxylation using microbial enzymes and whole-cell biocatalysts. In general, the regioselectivity of reaction was remarkably similar between the different catalysts in that little aromatic or benzylic, but significant aliphatic hydroxylation was observed. However, a more detailed investigation of isolated products showed complementary substrate specificity, functional group compatibility, and regioselectivity of hydroxylation. Substrate specificity and regioselectivity could be further modulated by small changes to the nature of the aromatic side chain, which appears to play an important role in substrate recognition, (C) 1999 Academic Press.