Behavior of failed bonded interfaces under in vitro cariogenic challenge.
Behavior of failed bonded interfaces under in vitro cariogenic challenge.
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DOI：
10.1016/j.dental.2016.02.005
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Huysmans MC
中科院分区：
文献类型：
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作者：
Montagner AF;Opdam NJ;Ruben JL;Bronkhorst EM;Cenci MS;Huysmans MC
本研究旨在比较不同复合体-牙本质界面上牙本质壁龋病的发生情况。使用两种粘接系统(酸洗和自酸蚀技术)，用复合树脂修复牙本质样本(10.4mm2)。产生了不同的有间隙的复合-牙本质界面：a)粘结失败，在提交老化方案(无老化、机械加载或水存储)后，界面处断裂；b)非粘结界面，既没有任何粘结材料，也没有粘结材料，或者只在牙本质上应用粘结材料。采用乳酸凝胶(pH=5)龋损模型，连续开放/闭合界面间隙10天，观察粘连断裂和未粘结的龋样。拍摄不依赖于波长的横向显微X线片，测量病变深度和矿物质丢失。数据采用线性混合效应回归模型进行分析。不同复合体-牙本质界面的龋病发生不同(p<；0.001)。粘结失败组牙本质无粘结界面的病变深度小于粘结失败组，未粘结界面的病变深度最深。粘接系统之间的差异仅在非粘结组中观察到(p=0.003)，应用于牙本质上的自酸蚀粘接剂显示出更严重的损伤。机械加载老化后断裂的样品比水储存后断裂的样品显示出更深的损伤(p<；0.001)。老化后失效的复合牙本质界面表现出与从未粘结的界面不同的脱矿作用，表明修复治疗在与继发性龋齿发展相关的方式上改变了组织。
This in vitro study aimed to compare dentin wall caries development at different composite-dentin interfaces. Dentin samples (10.4 mm2) were restored with composite resin using two adhesive systems (etch-and-rinse and self-etch techniques). Different composite-dentin interfaces with gaps were produced: a) failed bonded, which were fractured at interface after being submitted to ageing protocols (no ageing, mechanical loading or water storage); b) non-bonded interfaces, both without any adhesive material or with adhesive material applied only on the dentin. Adhesively fractured and non-bonded samples were subjected to a lactic acid gel (pH = 5) caries model with a continuous opening/closing movement of the interfacial gap for 10 days. Transverse wavelength-independent microradiographs were taken, and lesion depth and mineral loss were measured. Data were analyzed with linear mixed-effects regression models. Caries development differed among the composite-dentin interfaces (p < 0.001). The non-bonded interface with adhesive material on the dentin showed less lesion depth than the failed bonded groups, while the non-bonded interface without adhesive on dentin showed the deepest wall lesions. Difference between the adhesive systems was observed only in the non-bonded groups (p = 0.003), with the self-etch adhesive applied on the dentin showing more severe lesions. Samples broken after mechanical loading ageing showed deeper lesions than those broken after water storage (p < 0.001). Composite-dentin interfaces failed after ageing presented different demineralization from interfaces that were never bonded, indicating that the restorative treatment changes the tissue in a way relevant to secondary caries development.