Chemotactic activity for human PMN generated during ethanol metabolism by rat hepatocytes: role of glutathione and glutathione peroxidase.
Chemotactic activity for human PMN generated during ethanol metabolism by rat hepatocytes: role of glutathione and glutathione peroxidase.
复制标题
大鼠肝细胞乙醇代谢过程中产生的人 PMN 的趋化活性：谷胱甘肽和谷胱甘肽过氧化物酶的作用。
DOI：
10.1016/0006-291x(90)90646-5
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发表时间：
1990
影响因子：
3.1
通讯作者：
Roll,FJ
中科院分区：
文献类型：
--
作者：
Neuschwander-Tetri,BA;Roll,FJ
多形核白细胞浸润肝脏是酒精性肝炎的特征性表现。我们之前已经表明，肝细胞质代谢乙醇产生对多形核白细胞的化学引诱活性，羟基自由基清除剂抑制这一过程。为了研究内源性谷胱甘肽和谷胱甘肽过氧化物酶也抑制这一过程的可能性，我们评估了谷胱甘肽和谷胱甘肽过氧化物酶缺乏的大鼠肝细胞质在乙醇代谢过程中产生的化学引诱物活性。与谷胱甘肽和谷胱甘肽过氧化物酶含量正常的细胞质相比，将缺乏谷胱甘肽和谷胱甘肽过氧化物酶的细胞质与10毫米乙醇孵育1小时，其化学引诱活性增加500倍。这些发现为氧自由基在产生趋化活性中的作用提供了进一步的证据，它们还表明，其他人报道的乙醇诱导的肝谷胱甘肽和谷胱甘肽过氧化物酶的减少可能对促炎细胞进入肝脏的募集具有显著的增强作用。
Infiltration of the liver by polymorphonuclear leukocytes is a characteristic feature of alcoholic hepatitis. We have previously shown that liver cytosol metabolizing ethanol generates chemoattractant activity for polymorphonuclear leukocytes and that hydroxyl radical scavengers inhibit this process. To investigate the possibility that endogenous glutathione and glutathione peroxidase also inhibit this process, we evaluated chemoattractant activity production by glutathione and glutathione peroxidase deficient rat liver cytosol during ethanol metabolism. Incubation of cytosol deficient in both glutathione and glutathione peroxidase with 10 mM ethanol for 1 hour resulted in a 500-fold increase in chemoattractant activity when compared to cytosol with normal glutathione and glutathione peroxidase content. These findings provide further evidence for a role of oxygen free radicals in the generation of chemotactic activity and they also suggest that the ethanol-induced decrease in hepatic glutathione and glutathione peroxidase reported by others may have a significant potentiating effect on the recruitment of pro-inflammatory cells into the liver.