The new face of nucleolin in human melanoma
The new face of nucleolin in human melanoma
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DOI：
10.1007/s00262-009-0705-8
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发表时间：
2009-09-01
影响因子：
5.8
通讯作者：
Litynska, Anna
中科院分区：
文献类型：
--
作者：
Hoja-Lukowicz, Dorota;Przybylo, Malgorzata;Litynska, Anna
核仁素是一种多功能蛋白质，主要存在于核仁中，也存在于细胞质和质膜中。核外核仁素通过其糖基化而不同于核形式。对核仁素在乳腺癌中表达的研究表明，核仁素可能与转移级联反应有关。在本研究中，野豌豆凝集素（VVL）沉淀，随后进行聚丙烯酰胺凝胶电泳和质谱分析，证明核仁素是黑色素瘤中表达的VVL阳性糖蛋白。共聚焦显微镜证实了VVL阳性核仁素在黑色素瘤细胞膜和细胞质中的存在。使用生物信息学肽预测程序，核仁素被证明在其序列中含有多种可能的MHC I类结合肽，这使得核仁素成为一种有趣的黑色素瘤标记物和用于免疫诊断和可能的治疗目的的靶标。
Nucleolin is multifunctional protein mainly present in nucleoli but also detected in cytoplasm and plasma membranes. Extranuclear nucleolin differs from the nuclear form by its glycosylation. Studies on expression of nucleolin in breast cancer suggest a possible association to the metastatic cascade. In the present study, Vicia villosa lectin (VVL) precipitation followed by subsequent polyacrylamide gel electrophoresis and mass spectrometry analysis demonstrates nucleolin as a VVL-positive glycoprotein expressed in melanoma. The presence of VVL-positive nucleolin in the melanoma cell membrane and cytoplasm was confirmed by confocal microscopy. Using bioinformatic peptide prediction programs, nucleolin was shown to contain multiple possible MHC class-I binding peptides in its sequence which makes nucleolin an interesting melanoma marker and target for immunodiagnostic and possibly therapeutic purposes.