Resistance to the Bacillus sphaericus toxin in cultured mosquito cells.
Resistance to the Bacillus sphaericus toxin in cultured mosquito cells.
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培养的蚊子细胞对球形芽孢杆菌毒素的抵抗力。
DOI：
10.1007/bf02624000
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Davidson,EW
中科院分区：
文献类型：
--
作者：
Schroeder,JM;Chamberlain,C;Davidson,EW
用剂量递增的球形芽孢杆菌毒素从致倦库蚊中筛选出一株抗毒素的细胞系。该耐药细胞系已被证实为BEC。通过同工酶分析和核型分析研究了Ququiefasciatusin的来源。抗性品系与未选育的感病品系一样，结合了荧光标记的毒素。很快就达到了很高的阻力水平，并在4个月后保持了这一水平。在无毒素的情况下培养。
Increasing doses ofBacillus sphaericustoxin were used to select a toxin-resistant cell line from theCulex quinquefasciatusline. This resistant cell line was proven to beC. quinquefasciatusin origin by isozyme analysis and karyotype. The resistant line bound fluorescent-labeled toxin as did the unselected susceptible line. A high level of resistance was quickly achieved, and this level was maintained after 4 mo. culture in the absence of toxin.