Biologically active products of complement and acute lung injury in patients with the sepsis syndrome.
Biologically active products of complement and acute lung injury in patients with the sepsis syndrome.
复制标题
补体的生物活性产物和败血症综合征患者的急性肺损伤。
DOI：
10.1164/arrd.1984.130.5.791
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Murray,JF
中科院分区：
文献类型：
--
作者：
Weinberg,PF;Matthay,MA;Webster,RO;Roskos,KV;Goldstein,IM;Murray,JF
为了确定脓毒症时补体的生物活性产物是否存在，以及这些成分与脓毒症呼吸和血流动力学并发症的关系，我们测定了40例临床怀疑脓毒症患者血浆C5a-Des Arg和C3a-Des Arg(放射免疫法)、中性粒细胞趋化活性和中性粒细胞聚集活性。C3a-Des Arg和C5a-Des Arg分别在35例和38例患者中升高，在血培养阳性的25例患者中均升高。低血压(<90 mm Hg)和/或酸血症患者C5a-Des Arg水平最高。有中性粒细胞趋化活性的患者C5a Des Arg浓度显著高于无中性粒细胞趋化活性的患者。中性粒细胞聚集活性对补体激活的敏感性较低，仅有8例患者呈阳性，且与C5a Des Arg和C3a Des Arg值相关性较差。使用复合评分系统对脓毒症相关的肺异常进行量化，我们发现补体激活产物的生物学或免疫学检测既不与最初的严重程度相关，也不预测相关急性肺损伤的发展或恶化。
To determine if biologically active products of complement appear during sepsis and to establish the relationship of these components to the respiratory and hemodynamic complications of sepsis, we measured C5a des Arg and C3a des Arg (radioimmunoassay), neutrophil chemotaxis, and neutrophil-aggregating activity in plasma obtained from 40 patients at the time sepsis was suspected clinically. Levels of C3a des Arg and C5a des Arg were elevated in 35 and 38 patients, respectively, and in all 25 with positive blood cultures. Highest C5a des Arg levels occurred in patients with hypotension (less than 90 mmHg) and/or acidemia. The C5a des Arg concentrations were significantly higher in patients with than in those without neutrophil-chemotactic activity. Neutrophil-aggregating activity was less sensitive an index of complement activation, as it was positive in only 8 patients and correlated poorly with C5a des Arg and C3a des Arg values. Using a composite scoring system to quantify sepsis-related pulmonary abnormalities, we found that neither biologic nor immunologic assays of complement activation products correlated with the initial severity nor predicted the development or worsening of associated acute lung injury.