Binding of hematoporphyrin to synchronized cells from the line NHIK 3025.
Binding of hematoporphyrin to synchronized cells from the line NHIK 3025.
复制标题
血卟啉与来自 NHIK 3025 系的同步细胞的结合。
DOI：
10.1016/0304-3835(80)90113-5
复制
发表时间：
1980
期刊：
影响因子：
9.7
通讯作者：
J. Moan
中科院分区：
文献类型：
--
作者：
T. Christensen;J. Moan
用显微荧光法研究了血卟啉（HP）与人NHIK 3025细胞系的结合。HP在Hank平衡盐溶液中的浓度范围为10−5−3 × 10−3M时，会在细胞内浓缩。结合的时间过程是双相的，具有快速的初始结合和在长于30分钟的时间内较慢的积累。结合到HP的量随着细胞进行细胞周期而线性增加，并且从早期G1到晚期G2加倍。
The binding of hematoporphyrin (HP) to human cells of the line NHIK 3025 has been studied with the aid of microfluorometry. HP is concentrated within cells when present in Hank's balanced salt solution in the concentration range 10−5−3 × 10−3M. The time course of binding is biphasic with a rapid initial binding and a slower accumulation at times longer than 30 min. The amount bound to HP increases linearly as the cells proceed through the cell cycle and is doubled from early G1to late G2.