Tannase activity from the marine cyanobacterium Phormidium valderianum BDU140441
Tannase activity from the marine cyanobacterium Phormidium valderianum BDU140441
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海洋蓝藻 Phomidium valderianum BDU140441 的鞣酸酶活性
DOI：
10.1007/s10811-011-9738-4
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发表时间：
2012
影响因子：
3.3
通讯作者：
U. Lakshmanan
中科院分区：
文献类型：
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作者：
Swaminathan Palanisami;K. Kannan;U. Lakshmanan
海洋蓝藻phormidium valderianumdu 140441显示出在0.25 mM单宁酸下生长的能力，单宁酸是一种已知的阻碍微生物生长的化学物质。该生物的单宁酸降解能力从紫外可见吸收光谱可见。此外，通过活性染色确定了单宁酶的存在，并在天然凝胶中证实了其对单宁酸暴露的诱导活性。单宁酸代谢的关键酶是多酚氧化酶和酯酶；两者都以单宁酸降解而闻名。单宁酸暴露后，多酚氧化酶活性增加，并通过活性染色鉴定出一些新的酯酶异构体的表达。
The marine cyanobacteriumPhormidium valderianumBDU 140441 exhibited the ability to grow at 0.25 mM tannic acid, a known hindering chemical for microbial growth. The tannic acid-degrading ability of the organism is evident from the UV–visible absorption spectrum. In addition, the existence of tannase has been localized by activity staining, and its induction in activity upon tannic acid exposure was confirmed in native gel. The critical tannic acid metabolization enzymes tested for are polyphenol oxidase and esterases; both are well known for tannic acid degradation. Upon tannic acid exposure, increased activity of polyphenol oxidase and expression of few new isoforms of esterase were identified by activity staining.