Biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum for accelerating bone regeneration
Biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum for accelerating bone regeneration
复制标题
仿生有机-无机混合水凝胶静电纺丝骨膜加速骨再生
DOI：
10.1016/j.msec.2020.110670
复制
发表时间：
2020-05-01
影响因子：
7.9
通讯作者：
Cui, Wenguo
中科院分区：
文献类型：
--
作者：
Liu, Wenjuan;Bi, Wei;Cui, Wenguo
骨膜作为骨结构的重要组成部分，主要负责提供营养和调节成骨分化。当骨缺损发生时，骨膜的功能也会受到影响，最终会阻碍骨再生的过程。然而，制造能够加速缺损区域血管生成和成骨的人工骨膜仍然是研究人员面临的挑战。在本研究中，我们通过静电纺丝制备了一种有机-无机杂化仿生骨膜，它可以诱导原位矿化并长期控制局部区域的离子释放。此外，该系统在体外表现出潜在的促进能力，这意味着在体内加速骨再生的潜力。采用乳液法制备磷酸钙纳米粒子（CaPs），然后通过静电纺丝纤维将CaPs进一步与明胶甲基丙烯酰（GelMA）结合，构建杂化水凝胶纤维。纤维表现出令人满意的形态和机械性能，此外，可以观察到受控离子释放超过 10 天。此外，与模拟体液（SBF）共孵育7天和14天后，杂化纤维表面出现显着矿化。然后，通过与MC3T3-E1细胞共培养，证实了杂化纤维具有良好的生物相容性。最后，混合纤维通过与 HUVEC 和 MC3T3-E1 细胞共培养，表现出促进血管生成和骨生成的潜在能力。这种仿生有机-无机杂化水凝胶静电纺丝骨膜为开发具有血管生成和成骨能力的骨膜生物材料提供了一种有前途的策略。
Periosteum as an important component in the construct of bone is mainly responsible for providing nourishment and regulating osteogenic differentiation. When bone defect happens, the functionality of periosteum will also be influenced, furthermore, it will finally hamper the process of bone regeneration. However, fabrication of an artificial periosteum with the capabilities in accelerating angiogenesis and osteogenesis in the defect area is still a challenge for researchers. In this study, we fabricated an organic-inorganic hybrid biomimetic periosteum by electrospinning, which can induce mineralization in situ and control the ions release for long-term in local area. Further, this system exhibited potential capabilities in promoting in vitro, which means the potentiality in accelerating bone regeneration in vivo. Calcium phosphate nanoparticles (CaPs) were fabricated by emulsion method, then CaPs were further incorporated with gelatin-methacryloyl (GelMA) by electrospinning fibers to construct the hybrid hydrogel fibers. The fibers exhibited satisfactory morphology and mechanical properties, additionally, controlled ions release could be observed for over 10 days. Further, significant mineralization was proved on the surface of hybrid fibers after 7 days and 14 days' co-incubation with simulated body fluid (SBF). Then, favorable biocompatibility of the hybrid fibers was approved by co-cultured with MC3T3-E1 cells. Finally, the hybrid fibers exhibited potential capabilities in promoting angiogenesis and osteogenesis by co-culture with HUVECs and MC3T3-E1 cells. This biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum provided a promising strategy to develop periosteum biomaterials with angiogenesis and osteogenesis capabilities.