Spatial and temporal control of laminin-332 and-511 expressions during hair morphogenesis
Spatial and temporal control of laminin-332 and-511 expressions during hair morphogenesis
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DOI：
10.1007/s00795-013-0040-1
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发表时间：
2014-03-01
影响因子：
1.8
通讯作者：
Kishi, Kazuo
中科院分区：
文献类型：
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作者：
Imanishi, Hisayoshi;Tsuruta, Daisuke;Kishi, Kazuo
毛发是最小的器官之一，但对哺乳动物有许多重要的功能。毛发的形态发生是通过上皮和间质信号的相互交换发生的。关于lamin511和-332在毛发形态发生过程中的表达有一些报道，但关于lamin511及其反调控因子lamin332在毛发形态发生过程中的表达和功能尚未见报道。我们的免疫印迹结果显示，laminin-332蛋白在第0期被检测到，在第1 - 2期被下调，然后在第3期被恢复。然而，在整个0-3期，层粘连蛋白α 5的表达是恒定的。半定量RT-PCR结果显示，从0期到2期，所有laminin-332亚基mRNA表达量逐渐升高，而从0期到3期，所有laminin-511亚基mRNA表达量保持不变。我们的结果表明，适当表达laminin332和laminin511可能调节适当的毛发形态发生。
Hair is one of the smallest organs, but has many important functions to mammals. Hair morphogenesis occurs through the reciprocal exchange of epithelial and mesenchymal signals. There are some reports about the expression of laminin-511 and -332 during hair morphogenesis, but are no reports of the chronological expression and function of laminin-511 and its counter regulator laminin-332 during hair morphogenesis. Our results of immunoblotting revealed that laminin-332 proteins were detected at stage 0 and downregulated during stage 1 to stage 2, and then recovered at stage 3. However, laminin alpha 5 expression was constant throughout stages 0-3. According to the results of semi-quantitative RT-PCR, the mRNA expression of all laminin-332 subunits increased gradually from stage 0 to stage 2, while the mRNA expression of all laminin-511 subunits remained constant from stage 0 to stage 3. Our results suggest that the proper expression of laminin-332 and laminin-511 may regulate appropriate hair morphogenesis.