Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents
Effect of temperature on the chromatographic behavior of epirubicin and its analogues on high purity silica using reversed-phase solvents
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DOI：
10.1365/s10337-007-0200-3
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发表时间：
2007-05-01
期刊：
影响因子：
1.7
通讯作者：
Huang, Junxiong
中科院分区：
文献类型：
--
作者：
Dong, Lili;Huang, Junxiong
本文系统地研究了温度对表阿霉素及其类似物在反相溶剂高纯硅胶上保留行为的影响。发现温度对保留的影响高度依赖于有机改性剂的类型和浓度以及移动的相的pH。在富含有机物的移动的相中，有机改性剂的类型起重要作用。用非质子溶剂作为改性剂，保留时间随温度升高而异常增加。同时，效率和分辨率都得到显著提高，但这不是用非质子溶剂作为改性剂的情况。此外，温度对保留时间和选择性表现出不同的影响，当pH值变化时，温度依赖性的选择性反转被发现在较高的pH值。在富含水的移动的相中，无论有机溶剂的性质和pH值如何，溶质的保留都随着温度的升高而下降。似乎温度对溶质在裸二氧化硅上使用含有各种有机改性剂或pH的移动的相的色谱行为的影响是由许多不同的保留机制引起的。
The influence of temperature on the retention behavior of epirubicin and its analogues on high purity silica with reversed-phase solvents has been systematically investigated. it was found that temperature effects on retention are highly dependent on the type and concentration of organic modifier, as well as the pH of the mobile phase. In organic-rich mobile phases, the type of organic modifier plays an important role. With an aprotic solvent as modifier, retention times show anomalous increases with elevated temperature. At the same time, both efficiency and resolution are significantly improved but this is not the situation with aprotic solvent as modifier. In addition, temperature shows different effects on retention time and selectivity when the pH is changed, and temperature-dependent selectivity reversal is found at higher pHs. In aqueous-rich mobile phases, regardless of the nature of the organic solvent and pH, retention of solutes drops as temperature is raised. It seems that the effect of temperature on chromatographic behavior of the solutes on bare silica using mobile phases containing various organic modifiers or pHs, results from a number of different retention mechanisms.