High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories
High frame-rate multichannel beam-scanning microscopy based on Lissajous trajectories
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DOI：
10.1364/oe.22.024224
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发表时间：
2014-10-06
期刊：
影响因子：
3.8
通讯作者：
Simpson, Garth J.
中科院分区：
文献类型：
--
作者：
Sullivan, Shane Z.;Muir, Ryan D.;Simpson, Garth J.
描述了一种基于利萨如轨迹成像的简单光束扫描光学设计，用于在多个同时数据采集通道上实现高达 kHz 帧速率的光学成像。简而言之，两个快速扫描谐振镜将光束引导到通过视场的迂回轨迹上，轨迹重复时间由镜周期的最小公倍数给出。将原始时域数据切割成子轨迹，并结合基于模型的图像重建 (MBIR) 3D 修复算法，可实现远高于利萨如轨迹重复时间的有效帧速率。由于子轨迹和全轨迹成像只是分析相同数据的不同方法，因此同时获取分辨率相对较低的高帧率图像和高分辨率的低帧率图像。执行利萨如成像所需的光学硬件仅对现有光束扫描硬件进行较小的修改，并结合额外的控制和数据采集电子设备。基于激光透射率成像和偏振相关二次谐波发生显微镜的初步研究支持该方法对于检测大信号的细微变化和瞬态波动的痕量光检测的可行性。 (C) 2014年美国光学学会
A simple beam-scanning optical design based on Lissajous trajectory imaging is described for achieving up to kHz frame-rate optical imaging on multiple simultaneous data acquisition channels. In brief, two fast-scan resonant mirrors direct the optical beam on a circuitous trajectory through the field of view, with the trajectory repeat-time given by the least common multiplier of the mirror periods. Dicing the raw time-domain data into sub-trajectories combined with model-based image reconstruction (MBIR) 3D in-painting algorithms allows for effective frame-rates much higher than the repeat time of the Lissajous trajectory. Since sub-trajectory and full-trajectory imaging are simply different methods of analyzing the same data, both high-frame rate images with relatively low resolution and low frame rate images with high resolution are simultaneously acquired. The optical hardware required to perform Lissajous imaging represents only a minor modification to established beam-scanning hardware, combined with additional control and data acquisition electronics. Preliminary studies based on laser transmittance imaging and polarization-dependent second harmonic generation microscopy support the viability of the approach both for detection of subtle changes in large signals and for trace-light detection of transient fluctuations. (C) 2014 Optical Society of America