THE SIZE OF CHROMATIN LOOPS IN HELA-CELLS
THE SIZE OF CHROMATIN LOOPS IN HELA-CELLS
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DOI：
10.1002/j.1460-2075.1990.tb08144.x
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发表时间：
1990-02-01
期刊：
影响因子：
11.4
通讯作者：
COOK, PR
中科院分区：
文献类型：
--
作者：
JACKSON, DA;DICKINSON, P;COOK, PR
人们普遍认为，染色质纤维在间期被组织成环，环被认为是核功能的重要单位。然而，仍然没有直接的证据循环，与环大小的估计差异很大。这导致了这样的建议，即一些环，甚至所有的环，在分离过程中人为地产生，因为染色质很容易聚集。我们现在使用包封在琼脂糖中的HeLa细胞研究了分离程序对环大小的影响，以便于操作。各种衍生物（即细胞核、类核、基质和支架）中的环大小严重依赖于程序;它们的一些（或全部）环是伪影。使用最“生理”条件分离的衍生物中的环大小为86 kb;这在整个细胞周期中保持不变。该环大小可能是5至200 kb环范围的平均值。
It is widely believed that the chromatin fibre is organized into loops during interphase, with the loop being implicated as an important unit of nuclear function. However, there remains little direct evidence for looping, with estimates of loop size varying widely. This has led to the suggestion that some loops, or even all of them, arise artefactually during isolation as chromatin aggregates so easily. We have now investigated the effect of isolation procedure on loop size using HeLa cells encapsulated in agarose to allow easy manipulation. Loop size in various derivatives (i.e. nuclei, nucleoids, matrices ad scaffolds) critically depended upon procedure; some (or all) of their loops are artifacts. The loop size in derivatives isolated using the most ''physiological'' conditions was 86 kb; this remained unchanged throughout the cell cycle. This loop size is probably an average of a range of loops of between 5 and 200 kb.