DEVELOPMENT OF DISEASE AND VIRUS RECOVERY IN TRANSGENIC MICE CONTAINING HIV PROVIRAL DNA
DEVELOPMENT OF DISEASE AND VIRUS RECOVERY IN TRANSGENIC MICE CONTAINING HIV PROVIRAL DNA
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DOI：
10.1126/science.3201255
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发表时间：
1988-12-23
期刊：
影响因子：
56.9
通讯作者：
MARTIN, MA
中科院分区：
文献类型：
--
作者：
LEONARD, JM;ABRAMCZUK, JW;MARTIN, MA
构建了含有人类免疫缺陷病毒 (HIV) 前病毒 DNA 完整拷贝的转基因小鼠。始祖动物没有 HIV 病毒血症，并且在 9 个月的观察期内保持健康。与非转基因动物交配后，一只始祖小鼠（No. 13）产生了 F1 后代，该后代出现了一种疾病综合征，其特征是明显的表皮增生、淋巴结肿大、脾肿大、肺淋巴浸润、生长迟缓，并在出生后第 25 天死亡。通过免疫印迹分析，从五只受影响动物中的五只的脾脏、淋巴结和皮肤中发现了传染性艾滋病毒，与亲本病毒无法区分。
Transgenic mice containing intact copies of the human immunodeficiency virus (HIV) proviral DNA were constructed. Founder animals were not viremic for HIV and remained healthy during a 9-month observation period. After being mated with nontransgenic animals, one founder mouse (No. 13) gave rise to F1 progeny that developed a disease syndrome characterized by marked epidermal hyperplasia, lymphadenopathy, splenomegaly, pulmonary lymphoid infiltrates, growth retardation, and death by day 25 of life. Infectious HIV, indistinguishable from parental virus by immunoblot analysis, was recovered from the spleen, lymph nodes, and skin of five of five affected animals.