MAST-CELLS ARE NOT ESSENTIAL TO INFLAMMATION IN MURINE MODEL OF COLITIS
MAST-CELLS ARE NOT ESSENTIAL TO INFLAMMATION IN MURINE MODEL OF COLITIS
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肥大细胞对小鼠结肠炎模型中的炎症并不重要
DOI：
10.1007/bf02088336
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发表时间：
1994-03-01
影响因子：
3.1
通讯作者：
BARRETT, KE
中科院分区：
文献类型：
--
作者：
CHIN, KW;BARRETT, KE
一个简单的大鼠慢性肠道炎症模型，以确定是否肥大细胞在其诱导或延续发挥重要作用。通过直肠内施用溶于50%乙醇的三硝基苯磺酸在C57 BL小鼠中诱导结肠炎。 与大鼠相比，小鼠每克体重需要更高剂量的三硝基苯磺酸，有效剂量范围较窄（上限剂量受不可接受的死亡率限制，下限剂量受炎症减少限制）。给药小鼠的结肠肉眼可见发炎，伴透壁损伤、与相邻结构粘连和溃疡。通过组织重量和髓过氧化物酶含量对炎症进行主观评分，0.3-10 mg剂量的三硝基苯磺酸可使各指标呈剂量依赖性增加。 六毫克的三硝基苯磺酸诱导可再现的炎症长达四周。三硝基苯磺酸可以以类似的方式在肥大细胞缺陷型W/W（v）小鼠及其正常+/+同窝仔中诱导炎症。 因此，有可能在小鼠中诱导慢性结肠炎。肥大细胞不是这个过程的重要参与者。
A simple rat model of chronic intestinal inflammation was adapted to mice in order to ascertain whether mast cells play an essential role in its induction or perpetuation. Colitis was induced in C57BL mice by intrarectal administration of trinitrobenzene sulfonic acid in 50% ethanol. Higher doses of trinitrobenzene sulfonic acid per gram of body weight were required in mice than rats, with a narrower effective dose range (the upper dose limited by unacceptable mortality and the lower by decreased inflammation). Colons of treated mice were macroscopically inflamed, with transmural damage, adhesions to adjacent structures, and ulcerations. Inflammation was scored subjectively and by tissue weight and myeloperoxidase content, each index was increased dose-dependently by trinitrobenzene sulfonic acid doses of 0.3-10 mg. Six milligrams of trinitrobenzene sulfonic acid induced reproducible inflammation for up to four weeks. Trinitrobenzene sulfonic acid could induce inflammation in both mast-cell-deficient W/W(v) mice and their normal +/+ littermates in a similar fashion. Thus it is possible to induce chronic colitis in the mouse. Mast cells are not essential participants in this process.