Human rhinovirus serotypes in the nasal washes and mucosa of patients with chronic rhinosinusitis
Human rhinovirus serotypes in the nasal washes and mucosa of patients with chronic rhinosinusitis
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DOI：
10.1002/alr.21472
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发表时间：
2015-03-01
影响因子：
6.4
通讯作者：
Jang, Yong Ju
中科院分区：
文献类型：
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作者：
Lee, Sung Bu;Yi, Jong Sook;Jang, Yong Ju
慢性鼻窦炎（CRS）可因病毒性上呼吸道感染而加重.我们的目的是调查是否有任何特定的人鼻病毒（HRV）血清型是更常见的CRS患者的粘膜，并找到任何定义的临床特征，根据各种HRV serotypes.MethodsA前瞻性病例对照研究，以确定HRV血清型在111 CRS患者和51非CRS对照。没有参与者在4周内有上呼吸道感染史。前瞻性收集鼻灌洗液和鼻甲上皮细胞。当HRV检测与多重聚合酶链反应（PCR），菌株的特征进一步通过测序的HRV基因组的VP4/VP2区域。ResultsHRV检测40 CRS受试者（36%）和11非CRS对照（21%）。CRS组HRV总检出率高于非CRS组（p <0.05）。在CRS患者中检测到的8种血清型中，有5种属于HRV-A型，3种属于HRV-B型，未检测到HRV-C型。在非CRS对照中，仅鉴定出HRV-A，仅检测到2种血清型（HRV-A13和HRV-A16）。结论CRS患者中HRV的检出率较高。然而，我们无法确定某些HRV血清型是否在CRS患者中比非CRS对照组更占优势。HRV-A13是CRS患者和非CRS对照中最常见的血清型。CRS患者的HRV血清型在临床特征上无差异。
BackgroundChronic rhinosinusitis (CRS) can be aggravated by viral upper respiratory infections. We aimed to investigate whether any specific human rhinovirus (HRV) serotype is more common in the mucosa of CRS patients, and to find any defining clinical characteristics, according to the various HRV serotypes.MethodsA prospective case-control study was conducted to determine HRV serotypes in 111 CRS patients and 51 non-CRS controls. No participant had a history of upper respiratory infection over a 4-week period. Nasal lavage fluids and turbinate epithelial cells were collected prospectively. When HRV was detected with multiplex polymerase chain reaction (PCR), strains were further characterized by sequencing the VP4/VP2 region of the HRV genome.ResultsHRV was detected in 40 CRS subjects (36%) and 11 non-CRS controls (21%). The overall detection rates of HRV in CRS patients were higher than in non-CRS controls (p < 0.05). Of the 8 serotypes detected in CRS patients, 5 belonged to HRV-A and 3 belonged to HRV-B; HRV-C was not detected. In non-CRS controls, only HRV-A was identified, with only 2 serotypes detected (HRV-A13 and HRV-A16). HRV-B and C were not detected.ConclusionThe high prevalence of HRV in CRS patients was confirmed in our study. However, we were unable to determine whether certain HRV serotypes are more predominant in CRS patients than non-CRS controls. HRV-A13 was the most common serotype in both CRS patients and non-CRS controls. We could not find any differences in the clinical characteristics according to the HRV serotypes in CRS patients.