The development of deciduomata induced by two non-traumatic methods in the mouse.
The development of deciduomata induced by two non-traumatic methods in the mouse.
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通过两种非创伤性方法诱导小鼠蜕膜的发育。
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发表时间：
1968
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通讯作者：
C. Hetherington
中科院分区：
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作者：
C. Hetherington
在小鼠着床时，子宫对囊胚的存在做出反应，在基质中产生大量细胞反应，形成蜕膜瘤。蜕膜细胞反应可能启动的时期是相当明确的，并且该反应取决于卵巢激素对子宫的充分敏化。在这项研究中，采用了两种非创伤性的蜕膜瘤诱导方法，管腔内注射花生油（Finn＆Hinchliffe，1965）和管腔内注射空气，Orsini（1963）报道这对仓鼠和大鼠是有效的刺激。使用随机近交Q品系的处女小鼠，体重20p=n-25g，并且在与切除输精管的雄性交配后假怀孕。发现阴道塞之日为假孕第一天。第一个实验旨在发现子宫敏感性的发生时间和持续时间。治疗仅限于每个子宫的一个角，包括使用 30G 针和 0m= 微升注射器在假妊娠的前 5 天中的某一天（即 09.30 或 16.30 时）进行腔内注射 1ml 花生油，或在假孕时注射 2ml 空气。第3、4或5天p=m-每个实验组由五只小鼠组成。为了确定外伤性蜕膜瘤的程度，仅将注射器针头插入每个对照组的一只角。所有病例的尸检均在第 7 天的 11 时至 11 时 30 时之间进行，并在扭力天平上单独称重子宫角。在尸检中很明显，油和空气都发生了一些角间迁移，使得角之间的比较不可能，而对照角中的创伤性蜕膜很小或不存在。随着剂量的增加，油产生了更大的反应（文本-图 1）。数据表明，油诱导的敏感期持续时间较长，或更可能的是，油在管腔中停留的时间较长。如果将 0-02 毫升空气注入子宫角，则在生理盐水下解剖子宫时可检测到气泡长达 5 小时。在假妊娠第 4 天注射后，在 5 天内，子宫内可能会类似地检测到油。然而，假怀孕第一天注射的油只在五个经过处理的角之一中产生了一个小的蜕膜瘤。假怀孕第一天注射的油可能会从阴道漏出，而假怀孕第四天注射的油会被蜕膜困住。数据表明
In the mouse at implantation, the uterus responds to the presence of a blastocyst by producing a massive cellular reaction in the stroma to form a deciduoma. The period during which the decidual cell reaction may be initiated is fairly well defined, and the reaction depends on adequate sensitization of the uterus by the ovarian hormones. In this study, two non-traumatic methods of deciduoma induction were employed, intra-luminal injection of arachis oil (Finn & Hinchliffe, 1965), and intra-luminal injection of air, which was reported by Orsini (1963) to be an effective stimulus in the hamster and the rat. Virgin mice of the random outbred Q strain, weighing 20p=n-25 g, and pseudopregnant after mating with vasectomized males, were used. The day on which the vaginal plug was found was taken as the 1st day of pseudopregnancy. The first experiment was designed to discover the time of onset and duration of uterine sensitivity. The treatment, which was restricted to one horn of each uterus, consisted of an intraluminal injection, through the utero-tubal junction, using a 30G needle and a 0m=. 5ml microlitre syringe, of either 0m=. 1ml of arachis oil on one of the first 5 days of pseudopregnancy at 09.30 or 16.30 hours, or 0m=. 2ml of air on the 3rd, 4th or 5th dayp=m-eachexperimental group consisting of five mice. In order to determine the extent of traumatic deciduoma, a syringe needle was merely inserted into one horn of each of the control group. Autopsies, in all instances, were carried out between 11.00 and 11.30 hours on the 7th day, and the uterine horns were weighed individually on a torsion balance. It was apparent at autopsy that some inter-cornual migration of both oil and air had occurred, making a between-horn comparison impossible, while traumatic deciduomata in the control horns were small or absent. With the doses used, oil produced the greater response (Text-fig. 1). The data suggest either that the period of sensitivity for induction by oil is of longer duration or, more probably, that the oil remains longer in the lumen. If 0-02 ml of air is injected into a uterine horn, air bubbles may be detected, on dissecting the uterus under physiological saline, for up to 5 hr. Oil may be similarly detected in a uterus for 5 days, after injection on the 4th day of pseudopregnancy. Oil injected on the 1st day of pseudopregnancy, however, produced only one small deciduoma in one of five treated horns. Probably oil injected on the 1st day of pseudopregnancy is able to leak out of the vagina, while oil injected on the 4th day of pseudopregnancy is trapped by the deciduoma. The data suggest that