Water stress alters aphid-induced glucosinolate response in Brassica oleracea var. italica differently
Water stress alters aphid-induced glucosinolate response in Brassica oleracea var. italica differently
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DOI：
10.1007/s00049-011-0084-4
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发表时间：
2011-12-01
期刊：
影响因子：
1.8
通讯作者：
Mewis, Inga
中科院分区：
文献类型：
--
作者：
Khan, M. A. M.;Ulrichs, Christian;Mewis, Inga
芥子油苷（GS）是十字花科植物中主要的次生代谢产物，在植物防御和植物-昆虫通讯中发挥重要作用。植物中的 GS 生物合成及其积累可能受到环境中生物和非生物应激源的影响。在本研究中，西兰花植物 Brassica oleracea var. 中的 GS 水平。 italica Plenck 生长在不同的土壤水分条件下——浇水良好、干旱和涝渍——是在两种蚜虫——专科的 Brevicoryne brasicae (L.) 和通才 Myzus persicae (Sulzer) 取食 7 天后确定的。高效液相色谱分析表明，在不同水分状态条件下生长2周的意大利白菜植株上，甘蓝菜被B. 甘蓝植物取食后，GS水平显着升高。三种处理中的 GS 诱导之间没有确定显着差异。相比之下，桃蚜以植物为食后 GS 的诱导很大程度上取决于水分胁迫水平。浇水良好的植物中 GS 含量的增加比干旱条件下生长的植物更显着。当植物在浸水条件下生长时，饲喂桃花霉并不会增加 GS 水平。在所有三种水处理中，在桃叶霉侵染的植物中发现植物生长最低，并且GS的测量表明，桃叶霉对GS的诱导随着受侵染植物生长的减少而减少。甘蓝芽孢杆菌的 GS 诱导并没有随着水分胁迫下植物生长的变化而显着改变。在检测到的五种芥子油苷中，只有一种，即脂肪族 GS 4-甲基亚磺酰丁基，在给处于水分胁迫的植物上的桃 M. persicae 和 B. brasicae 中后保持不变。 GS水平的变化主要是由于蚜虫食草引起的吲哚基GS的诱导而观察到的，但吲哚-3-基甲基GS的变化并不总是与其他吲哚基GS的诱导一致。
Glucosinolates (GS) are the main secondary metabolites in brassicaceous plants that play an important role in plant defense and plant-insect communication. GS biosynthesis in plants and their accumulation may be influenced by biotic and abiotic stressors from the environment. In the present study, GS levels in broccoli plants, Brassica oleracea var. italica Plenck, grown under different water status conditions of soil-well-watered, drought and waterlogged-were determined after two aphid species, the specialist Brevicoryne brassicae (L.) and the generalist Myzus persicae (Sulzer), had fed on them for 7 days. High-performance liquid chromatography analysis revealed that GS levels were significantly induced after B. brassicae had fed on B. italica plants grown for 2 weeks under the various water status conditions. No significant differences were determined between GS induction in the three treatments. In contrast, the induction of GS after M. persicae had fed on the plants depended greatly on water stress levels. GS content in well-watered plants increased more significantly than in plants grown under drought conditions. Feeding by M. persicae did not increase GS levels when plants were grown under waterlogged conditions. The lowest plant growth were found in M. persicae-infested plants for all three water treatments, and measurements of GS showed that the induction of GS by M. persicae decreased in line with the reduction in infested plant growth. GS induction by B. brassicae did not alter remarkably following change in plant growth under water stress. Only one of the five glucosinolates detected, the aliphatic GS 4-methylsulfinylbutyl, remained unchanged after feeding of both, M. persicae and B. brassicae, on plants under water stress. Variation in GS levels was mainly observed for the induction of indolyl GS due to aphid herbivory, but changes in indol-3-ylmethyl GS were not always consistent with the induction of other indolyl GS.