Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
Integrative Analyses of mRNA Expression Profile Reveal the Involvement of IGF2BP1 in Chicken Adipogenesis
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mRNA 表达谱的综合分析揭示了 IGF2BP1 在鸡脂肪形成中的作用
DOI：
10.3390/ijms20122923
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发表时间：
2019-06-02
影响因子：
5.6
通讯作者：
Luo, Wen
中科院分区：
文献类型：
--
作者：
Chen, Jiahui;Ren, Xueyi;Luo, Wen
腹脂沉积过多是肉鸡生产中普遍关注的问题，尤其是我国地方鸡种。高脂饮食可导致体重增加和脂肪沉积过多，影响脂肪代谢和脂肪形成的基因和途径。为了揭示影响鸡腹脂沉积的主要基因和途径，本研究分别采用高脂饲料和普通饲料饲喂中国地方鸡种。结果表明，高脂饮食可增加腹部脂肪沉积，诱导脂肪细胞肥大。此外，我们使用RNA测序来鉴定HFD和ND鸡肝脏和腹部脂肪中的差异表达基因（DEG）。通过对这些DEG的分析，我们发现许多DEG都富含脂肪代谢相关的途径，如过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor，PPAR）信号传导、脂肪消化吸收、细胞外基质（extracellular matrix，ECM）-受体相互作用以及类固醇激素的生物合成。值得注意的是，胰岛素样生长因子II mRNA结合蛋白1（IGF 2BP 1）的表达，这是一种IGF 2 mRNA的结合蛋白，被发现在肝脏和腹部脂肪诱导HFD。IGF 2BP 1在鸡肝相关细胞系Leghorn strain M鸡肝癌（LMH）细胞中的异位表达揭示了IGF 2BP 1可以调节脂肪酸代谢相关基因的表达。在鸡前脂肪细胞（ICP细胞系）中，我们发现IGF 2BP 1可以促进脂肪细胞的增殖和分化，并且IGF 2BP 1的过表达会增加脂滴含量。综上所述，本研究为了解中国地方肉鸡腹脂沉积相关基因和途径提供了新的思路，IGF 2BP 1是研究鸡脂肪代谢和脂肪形成的重要候选基因。
Excessive abdominal fat deposition is an issue with general concern in broiler production, especially for Chinese native chicken breeds. A high-fat diet (HFD) can induce body weight gained and excessive fat deposition, and genes and pathways participate in fat metabolism and adipogenesis would be influenced by HFD. In order to reveal the main genes and pathways involved in chicken abdominal fat deposition, we used HFD and normal diet (ND) to feed a Chinese native chicken breed, respectively. Results showed that HFD can increase abdominal fat deposition and induce adipocyte hypertrophy. Additionally, we used RNA-sequencing to identify the differentially expressed genes (DEGs) between HFD and ND chickens in liver and abdominal fat. By analyzed these DEGs, we found that the many DEGs were enriched in fat metabolism related pathways, such as peroxisome proliferator-activated receptor (PPAR) signaling, fat digestion and absorption, extracellular matrix (ECM)-receptor interaction, and steroid hormone biosynthesis. Notably, the expression of insulin-like growth factor II mRNA binding protein 1 (IGF2BP1), which is a binding protein of IGF2 mRNA, was found to be induced in liver and abdominal fat by HFD. Ectopic expression of IGF2BP1 in chicken liver-related cell line Leghorn strain M chicken hepatoma (LMH) cell revealed that IGF2BP1 can regulate the expression of genes associated with fatty acid metabolism. In chicken preadipocytes (ICP cell line), we found that IGF2BP1 can promote adipocyte proliferation and differentiation, and the lipid droplet content would be increased by overexpression of IGF2BP1. Taken together, this study provides new insights into understanding the genes and pathways involved in abdominal fat deposition of Chinese native broiler, and IGF2BP1 is an important candidate gene for the study of fat metabolism and adipogenesis in chicken.