EXPRESSION OF EPSTEIN-BARR VIRUS-ENCODED SMALL RNA (BY THE EBER-1 GENE) IN LIVER SPECIMENS FROM TRANSPLANT RECIPIENTS WITH POSTTRANSPLANTATION LYMPHOPROLIFERATIVE DISEASE
EXPRESSION OF EPSTEIN-BARR VIRUS-ENCODED SMALL RNA (BY THE EBER-1 GENE) IN LIVER SPECIMENS FROM TRANSPLANT RECIPIENTS WITH POSTTRANSPLANTATION LYMPHOPROLIFERATIVE DISEASE
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DOI：
10.1056/nejm199212103272403
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发表时间：
1992-12-10
影响因子：
158.5
通讯作者：
WEISS, LM
中科院分区：
文献类型：
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作者：
RANDHAWA, PS;JAFFE, R;WEISS, LM
背景EB病毒（EBV）相关的移植后淋巴组织增生性疾病（PTLD）发生在1%至10%的移植受者中，可以通过降低免疫抑制水平来治疗。我们假设在潜伏性EBV感染期间EBER-1基因转录的小RNA的组织表达将识别PTLD风险的患者。我们研究了EBER-1基因的表达，从24例患者的肝脏标本2天至22个月前的PTLD的发展，使用原位杂交与寡核苷酸探针。对照标本取自20例因器官获取、急性移植物排斥反应或病毒性肝炎而出现损伤体征的同种异体移植受者，这些受者在活检后9至71个月未发生PTLD。在24名PTLD患者中，17名（71%）的标本中有1%至40%的单核细胞对EBER-1基因呈阳性。此外，这17名患者中有10名（59%）的标本具有提示EBV肝炎的组织病理学变化。在每种情况下，EBER-1阳性细胞被发现在尸检中确定的淋巴增生性病变。20个对照组中只有2个（10%）的标本有EBER-1阳性细胞（P
Background. Epstein-Barr virus (EBV)-associated post-transplantation lymphoproliferative disease (PTLD) develops in 1 to 10 percent of transplant recipients, in whom it can be treated by a reduction in the level of immunosuppression. We postulated that the tissue expression of the small RNA transcribed by the EBER-1 gene during latent EBV infection would identify patients at risk for PTLD.Methods. We studied EBER-1 gene expression in liver specimens obtained from 24 patients 2 days to 22 months before the development of PTLD, using in situ hybridization with an oligonucleotide probe. Control specimens were obtained from 20 recipients of allografts with signs of injury due to organ retrieval, acute graft rejection, or viral hepatitis in whom PTLD had not developed 9 to 71 months after the biopsy.Results. Of the 24 patients with PTLD, 17 (71 percent) had specimens in which 1 to 40 percent of mononuclear cells were positive for the EBER-1 gene. In addition, 10 of these 17 patients (59 percent) had specimens with histopathological changes suggestive of EBV hepatitis. In every case, EBER-1-positive cells were found within the lymphoproliferative lesions identified at autopsy. Only 2 of the 20 controls (10 percent) had specimens with EBER-1-positive cells (P