MCT1 and its accessory protein CD147 are differentially regulated by TSH in rat thyroid cells
MCT1 and its accessory protein CD147 are differentially regulated by TSH in rat thyroid cells
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DOI：
10.1152/ajpendo.00172.2003
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发表时间：
2003-12-01
影响因子：
5.1
通讯作者：
Philp, NJ
中科院分区：
文献类型：
--
作者：
Fanelli, A;Grollman, EF;Philp, NJ
在甲状腺细胞中，基础糖酵解和TSH刺激的糖酵解与乳酸流出有关。在这份报告中，我们解决了是否单羧酸转运蛋白（MCT）存在于甲状腺组织输出过量的乳酸产生的有氧糖酵解。使用免疫染色技术，我们发现，MCT 4与其辅助蛋白CD 147定位在大鼠甲状腺滤泡细胞的基底外侧膜。在培养的大鼠甲状腺（FRTL-5）细胞中，表达MCT 1而非MCT 4。CD 147与MCT 1共定位和共免疫沉淀。TSH通过cAMP依赖性机制上调MCT 1/CD 147表达作为时间的函数，因为毛喉素再现TSH的作用。促甲状腺激素增强FRTL-5细胞中MCT 1和CD 147的蛋白表达。而MCT 1蛋白的表达是在转录水平控制，CD 147蛋白的表达是由转录后机制。这些研究的结果表明，激素刺激运输介导的调节MCT 1转录。
In thyroid cells, basal and TSH-stimulated glycolysis is associated with lactic acid efflux. In this report, we address whether monocarboxylate transporters (MCTs) are present in thyroid tissue for exporting excess lactic acid generated by aerobic glycolysis. Using immunostaining techniques, we show that MCT4 localizes with its accessory protein CD147 in the basolateral membrane of rat thyroid follicular cells. In cultured rat thyroid (FRTL-5) cells, MCT1 rather than MCT4 is expressed. CD147 colocalizes and coimmunoprecipitates with MCT1. TSH upregulates MCT1/CD147 expression as a function of time through a cAMP-dependent mechanism as forskolin reproduces the effect of TSH. TSH enhances protein expression of both MCT1 and CD147 in FRTL-5 cells. Whereas MCT1 protein expression is controlled at the level of transcription, CD147 protein expression is regulated by a posttranscriptional mechanism. Results of these studies suggest that hormone stimulation of transport is mediated by regulating MCT1 transcription.