"Do-It-Yourself" reliable pH-stat device by using open-source software, inexpensive hardware and available laboratory equipment.
"Do-It-Yourself" reliable pH-stat device by using open-source software, inexpensive hardware and available laboratory equipment.
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DOI：
10.1371/journal.pone.0193744
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Kostic M
中科院分区：
文献类型：
--
作者：
Milanovic JZ;Milanovic P;Kragic R;Kostic M
本文介绍了如何利用开源的“OpenPhControl”软件、廉价的硬件(一台蠕动、一台注射泵、一台步进电机…)，来构建一种可靠、廉价的pH调节装置。，现成的实验室设备：酸碱仪、计算机、网络摄像头和一些3D打印部件。我们为设备的每个部分以及整个系统的设计、开发和测试结果提供了一种方法。除了通过低成本蠕动泵给药外，我们还介绍了使用开源注射器泵对试剂进行仔细控制的给药。从泵控制和更大注射器的应用两个方面介绍了对基本开源注射器泵的升级。除了pH调节器的基本功能，即pH值的测量和维护外，还对该装置进行了改进，使其可以用于电位滴定。我们已经证明了该装置在用2，2，6，6-四甲基哌啶-1-氧自由基氧化纤维素纤维时的实用性，即用于TEMPO介导的氧化。为了支持这一点，我们给出了氧化动力学、添加试剂的消耗和实验重复性的结果。考虑到每个人都可以使用开源科学工具，而且研究人员可以根据需要建造和调整装置，而且不包括现有实验室设备(酸碱仪、计算机和词汇表)的开源酸碱仪装置的总成本不到150欧元，我们相信，以现有商业报价的一小部分成本，我们的开源酸碱仪可以显著改进需要使用酸碱仪的实验工作。
In this paper, we present the construction of a reliable and inexpensive pH stat device, by using open-source “OpenPhControl” software, inexpensive hardware (a peristaltic and a syringe pump, Arduino, a step motor…), readily available laboratory devices: a pH meter, a computer, a webcam, and some 3D printed parts. We provide a methodology for the design, development and test results of each part of the device, as well as of the entire system. In addition to dosing reagents by means of a low-cost peristaltic pump, we also present carefully controlled dosing of reagents by an open-source syringe pump. The upgrading of the basic open-source syringe pump is given in terms of pump control and application of a larger syringe. In addition to the basic functions of pH stat, i.e. pH value measurement and maintenance, an improvement allowing the device to be used for potentiometric titration has been made as well. We have demonstrated the device’s utility when applied for cellulose fibers oxidation with 2,2,6,6-tetramethylpiperidine-1-oxyl radical, i.e. for TEMPO-mediated oxidation. In support of this, we present the results obtained for the oxidation kinetics, the consumption of added reagent and experimental repeatability. Considering that the open-source scientific tools are available to everyone, and that researchers can construct and adjust the device according to their needs, as well as, that the total cost of the open-source pH stat device, excluding the existing laboratory equipment (pH meter, computer and glossary) was less than 150 EUR, we believe that, at a small fraction of the cost of available commercial offers, our open-source pH stat can significantly improve experimental work where the use of pH stat is necessary.
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