S1 ribosomal protein and the interplay between translation and mRNA decay
S1 ribosomal protein and the interplay between translation and mRNA decay
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DOI：
10.1093/nar/gkr417
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发表时间：
2011-09-01
影响因子：
14.9
通讯作者：
Briani, Federica
中科院分区：
文献类型：
--
作者：
Delvillani, Francesco;Papiani, Giulia;Briani, Federica
S1是一种与30S亚基弱相关的“非典型”核糖体蛋白，其参与翻译、转录和控制RNA稳定性。S1被认为通过协助30S在翻译起始区的定位参与翻译起始复合物的形成，但对其在其他RNA处理中的作用知之甚少。在这项工作中，我们分析了在体内不同的细胞内S1浓度的影响，从耗尽过度表达，翻译，衰减和细胞内分布的leadered和leadless信使RNA（mRNA）。我们表明，cspE mRNA，如rpsO转录，可以被切割的RNase E在多个站点，而leaderless cspE转录也可以通过一个替代途径降解由一个未知的内切核酸酶。在S1过表达时，cspE和rpsO mRNA的RNase E依赖性衰变被抑制，并且这些转录物被稳定，而未鉴定的核酸内切酶对无前导cspE mRNA的切割不受影响。总的来说，我们的数据表明，核糖体未结合的S1可能会抑制翻译，大肠杆菌核糖体的一部分可能实际上缺乏S1。
S1 is an 'atypical' ribosomal protein weakly associated with the 30S subunit that has been implicated in translation, transcription and control of RNA stability. S1 is thought to participate in translation initiation complex formation by assisting 30S positioning in the translation initiation region, but little is known about its role in other RNA transactions. In this work, we have analysed in vivo the effects of different intracellular S1 concentrations, from depletion to overexpression, on translation, decay and intracellular distribution of leadered and leaderless messenger RNAs (mRNAs). We show that the cspE mRNA, like the rpsO transcript, may be cleaved by RNase E at multiple sites, whereas the leaderless cspE transcript may also be degraded via an alternative pathway by an unknown endonuclease. Upon S1 overexpression, RNase E-dependent decay of both cspE and rpsO mRNAs is suppressed and these transcripts are stabilized, whereas cleavage of leaderless cspE mRNA by the unidentified endonuclease is not affected. Overall, our data suggest that ribosome-unbound S1 may inhibit translation and that part of the Escherichia coli ribosomes may actually lack S1.