REPERFUSION-INDUCED INJURY TO THE BLOOD-BRAIN-BARRIER AFTER MIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS
REPERFUSION-INDUCED INJURY TO THE BLOOD-BRAIN-BARRIER AFTER MIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS
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DOI：
10.1161/01.str.25.8.1658
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发表时间：
1994-08-01
期刊：
影响因子：
8.3
通讯作者：
BETZ, AL
中科院分区：
文献类型：
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作者：
YANG, GY;BETZ, AL
背景和目的血脑屏障的完整性在脑缺血后再灌注过程中可能发挥重要的病理生理作用。为探讨再灌注加重脑损伤的因素，本研究观察了大鼠大脑中动脉永久性或暂时性闭塞后脑血流量、血脑屏障通透性、水肿形成和梗死的变化，并研究了局部脑血流量与血脑屏障破坏的关系。允许永久（6小时）或暂时闭塞（3小时闭塞和3小时再灌注）。我们测量脑水，离子含量，梗死体积和确定脑血流量，用激光多普勒血流仪和血脑屏障通透性与[H-3] α-aminoisobutyricacid.Results闭塞期间，脑血流量减少到7%至15%（永久）和10%至17%（暂时）的基线。在再灌注3小时期间，它恢复到基线的47%至80%（外侧皮质）和78%至98%（内侧皮质）。与对侧半球相比，永久性和暂时性组缺血区脑组织含水量均增加（P
Background and Purpose The integrity of the blood-brain barrier may play an important pathophysiological role during postischemic reperfusion. To determine the factors that lead to exacerbation of brain injury by reperfusion, we investigated changes in cerebral blood flow, blood-brain barrier permeability, edema formation, and infarction in permanent or temporary middle cerebral artery occlusion in rats and studied the relation between local cerebral blood flow and blood-brain barrier disruption.Methods Middle cerebral artery occlusion was performed with the rat suture model, allowing either permanent (6 hours) or temporary occlusion (3 hours of occlusion and 3 hours of reperfusion). We measured brain water, ion contents, and infarct volumes and determined cerebral blood flow using laser Doppler flowmetry and blood-brain barrier permeability with [H-3]alpha-aminoisobutyric acid.Results During occlusion, cerebral blood flow was reduced to 7% to 15% (permanent) and 10% to 17% (temporary) of the baseline. During 3 hours of reperfusion, it returned to 47% to 80% (lateral cortex) and 78% to 98% (medial cortex) of the baseline. Compared with the contralateral hemisphere, the water content in the ischemic area increased in both permanent and temporary groups (P