Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas
Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas
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DOI：
10.1177/0300985810389312
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发表时间：
2011-01-01
影响因子：
2.4
通讯作者：
Nakayama, H.
中科院分区：
文献类型：
--
作者：
Ide, T.;Uchida, K.;Nakayama, H.
肿瘤细胞侵入周围神经组织是间变性脑膜瘤的组织学特征之一。为了确定其他可能的标记物的侵略犬脑膜瘤，组织学特征和参与细胞粘附，细胞增殖和侵袭的分子的表达之间的关系进行了检查。对55例犬脑膜瘤进行上皮钙粘蛋白（E-cadherin）、神经钙粘蛋白（N-cadherin）、β-连环蛋白、双皮质素（DCX）和Ki-67的免疫组化。DCX优先表达于侵犯脑实质的肿瘤细胞（14例中的12例），表明其参与了侵袭过程。无论组织学类型，E-cadherin和N-cadherin的表达分别在31/55和44/55例。DCX与N-cadherin表达呈显著正相关，E-cadherin与N-cadherin表达呈显著负相关，提示E-cadherin表达降低和N-cadherin表达升高可诱导DCX表达。55例中10例观察到典型的膜β-连环蛋白表达，而33例观察到核转位。在间变性脑膜瘤中经常发现核β-连环蛋白表达（14例中的12例）。Ki-67标记指数在间变性脑膜瘤中显著高于其他类型。这些结果表明，N-钙粘蛋白和DCX的表达和β-连环蛋白的核转位与脑膜瘤的侵袭和间变性密切相关。值得注意的是，颗粒细胞脑膜瘤对几乎所有检测的分子都是阴性的，这表明它们具有与其他脑膜瘤不同的肿瘤生物学。
Tumor cell invasion into the surrounding nervous tissue is one of the histologic hallmarks of anaplastic meningiomas. To identify other possible markers for aggression in canine meningiomas, the relationship between histologic features and the expression of molecules involved in cell adhesion, cell proliferation, and invasion was examined. Immunohistochemistry for epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), beta-catenin, doublecortin (DCX), and Ki-67 was performed for 55 cases of canine meningioma. DCX was preferentially expressed in tumor cells invading the brain parenchyma (12 of 14 cases), suggesting its involvement in the invasion process. Regardless of the histologic type, E-cadherin and N-cadherin expression was observed in 31 of 55 and 44 of 55 cases, respectively. There was a significant positive correlation between DCX and N-cadherin expression and a significant negative correlation between E-cadherin and N-cadherin expression, suggesting that decreased E-cadherin and increased N-cadherin expression induce DCX expression. Typical membranous beta-catenin expression was observed in 10 of 55 cases, whereas nuclear translocation was observed in 33 cases. Nuclear beta-catenin expression was frequently found in anaplastic meningiomas (12 of 14 cases). The Ki-67 labeling indices were significantly higher in anaplastic meningiomas than in other types. These findings indicate that the expression of N-cadherin and DCX and the nuclear translocation of beta-catenin are closely associated with the presence of invasion and anaplasia in canine meningiomas. Notably, granular cell meningiomas were negative for almost all the molecules examined, suggesting that they have a different tumor biology than other meningiomas.