Expression of free fatty acid receptor GPR40 in the central nervous system of adult monkeys
Expression of free fatty acid receptor GPR40 in the central nervous system of adult monkeys
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DOI：
10.1016/j.neures.2007.05.001
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发表时间：
2007-08-01
影响因子：
2.9
通讯作者：
Yamashima, Tetsumori
中科院分区：
文献类型：
--
作者：
Ma, Dexuan;Tao, Bangbao;Yamashima, Tetsumori
g蛋白偶联受体40 （GRP40）是游离脂肪酸的跨膜受体，因其与胰腺胰岛素分泌的关系而闻名。最近的研究表明，通过膳食补充花生四烯酸和/或二十二碳六烯酸，可以改善啮齿动物的空间记忆和海马长期增强以及人类的认知功能，这些酸可能是GPR40的配体。虽然游离脂肪酸对大脑的影响可能与GPR40的激活有关，但GPR40在中枢神经系统（CNS）中的作用目前尚不清楚。本研究采用免疫印迹和免疫组织化学方法研究了GPR40在成年猴中枢神经系统中的表达和分布。免疫印迹分析显示约31 kDa的条带与GPR40蛋白的大小一致。在大脑皮层、海马、杏仁核、下丘脑、小脑、脊髓等多种神经元的细胞核和/或核周中观察到GPR40的免疫反应性。此外，脑白质星形胶质细胞、大脑皮层分子层和多形层、沿侧脑室前角的室下区、海马齿状回颗粒下区均显示GPR40免疫反应性。本研究首先为阐明GPR40在灵长类动物中枢神经系统中的作用提供了形态学基础。(c) 2007爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
The G-protein coupled receptor 40 (GRP40) is a transmembrane receptor for free fatty acids, and is known for its relation to insulin secretion in the pancreas. Recent studies demonstrated that spatial memory and hippocampal lontg-term potentiation of rodents and cognitive function of humans are improved by a dietary supplementation with arachidonic and/or docosahexaenoic acids, which are possible ligands for GPR40. While free fatty acid effects on the brain might be related to GPR40 activation, the role of GPR40 in the central nervous system (CNS) is at present not known. Here, we studied expression and distribution of GPR40 in CNS of adult monkeys by immunoblotting and immunohistochemistry. Immunoblotting analysis showed a band of approximately 31 kDa consistent with the size of GPR40 protein. GPR40 immunoreactivity of was observed in the nuclei and/or perikarya of a wide variety of neurons including neurons in the cerebral cortex, hippocampus, amygdala, hypothalamus, cerebellum, spinal cord. In addition, astrocytes of the cerebral white matter, the molecular layer and multiform layer of the cerebral cortex, the subventricular zone along the anterior horn of the lateral ventricle, and the subgranular zone of the hippocampal dentate gyrus showed GPR40 immunoreactivity. The present data first provide a morphological basis for clarifying the role of GPR40 in the primate CNS. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.