Maestro of regulation: Riboswitches orchestrate gene expression at the levels of translation, transcription and mRNA decay
Maestro of regulation: Riboswitches orchestrate gene expression at the levels of translation, transcription and mRNA decay
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DOI：
10.1080/15476286.2018.1451721
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发表时间：
2018-01-01
期刊：
影响因子：
4.1
通讯作者：
Lafontaine, Daniel A.
中科院分区：
文献类型：
--
作者：
Bastet, Laurene;Turcotte, Pierre;Lafontaine, Daniel A.
核糖开关是 RNA 调节因子，通过响应代谢物结合调节基因结构来控制基因表达。研究核糖开关调节基因表达的机制对于理解核糖开关如何参与维持细胞稳态至关重要。先前的报告表明核糖开关可以在翻译、转录或 mRNA 衰减水平上控制基因表达。然而，很少有核糖开关调节基因表达多个步骤的例子。最近对翻译调节、TPP 依赖性核糖开关的研究表明，配体结合也参与 mRNA 水平的控制。在此模型中，TPP 与核糖开关的结合导致翻译抑制，进而导致 Rho 依赖性转录终止。因此，mRNA 水平是通过核糖体占据间接控制的。这与其他核糖开关相反，其他核糖开关通过调节参与 Rho 依赖性转录终止或 RNase E 裂解活性的调控序列的访问来直接控制 mRNA 水平。总之，这些发现表明核糖开关使用多种指导基因表达结果的策略来调节翻译起始和 mRNA 水平。
Riboswitches are RNA regulators that control gene expression by modulating their structure in response to metabolite binding. The study of mechanisms by which riboswitches modulate gene expression is crucial to understand how riboswitches are involved in maintaining cellular homeostasis. Previous reports indicate that riboswitches can control gene expression at the level of translation, transcription or mRNA decay. However, there are very few described examples where riboswitches regulate multiple steps in gene expression. Recent studies of a translation-regulating, TPP-dependent riboswitch have revealed that ligand binding is also involved in the control of mRNA levels. In this model, TPP binding to the riboswitch leads to the inhibition of translation, which in turn allows for Rho-dependent transcription termination. Thus, mRNA levels are indirectly controlled through ribosome occupancy. This is in contrast to other riboswitches that directly control mRNA levels by modulating the access of regulatory sequences involved in either Rho-dependent transcription termination or RNase E cleavage activity. Together, these findings indicate that riboswitches modulate both translation initiation and mRNA levels using multiple strategies that direct the outcome of gene expression.