The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway.
The in ovo injection of methionine improves intestinal cell proliferation and differentiation in chick embryos by activating the JAK2/STAT3 signaling pathway.
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DOI：
10.1016/j.aninu.2021.03.009
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Gao CQ
中科院分区：
文献类型：
--
作者：
Chen MJ;Zhou JY;Chen YJ;Wang XQ;Yan HC;Gao CQ
鸡胚的肠道健康对其终身生长至关重要，外源性营养干预可为胚胎发育提供充足的营养。本研究探讨了卵内注射L-蛋氨酸（L-Met）对鸡胚肠道结构和屏障功能的影响。试验分为4组：对照组（CON）注射磷酸盐缓冲液（PBS），其余3组分别注射5、10和20 mg L-Met/蛋。在胚胎第9天（E9）进行注射，并在孵化当天收集肠样品用于分析。结果表明，与CON组相比，卵内注射L-Met组的十二指肠、空肠和回肠的相对重量增加（P < 0.05）。苏木精-伊红（H&E）染色显示，注射5、10、20 mg L-Met组绒毛高度和腺窝深度显著增加（P < 0.05）。此外，10 mg L-Met还可增加空肠的跨上皮电阻（TEER）（P < 0.05）。注射10和20 mg L-Met后，紧密连接蛋白ZO-1和claudin-1的表达以及Ki 67和villin蛋白的荧光信号强度均显著增加（P < 0.05）。注射10和20 mg L-Met后，磷酸化Janus激酶2（p-JAK 2）和磷酸化信号转导子和转录激活子3（p-STAT 3）的蛋白表达显著增加（P < 0.05）。总之，注射L-Met，特别是在10 mg的剂量下，由于JAK 2/STAT 3信号通路的激活，对鸡胚的肠道完整性显示出有益的作用。本研究结果为调控鸡胚肠道发育和孵化后雏鸡快速生长提供了新的思路。
The intestinal health of chick embryos is vital for their life-long growth, and exogenous nutrition intervention may provide sufficient nutrition for embryonic development. In the present study, we investigated the effect of in ovo injection of L-methionine (L-Met) on the intestinal structure and barrier function of chick embryos. There were 4 groups of treatments: the control (CON) group injected with phosphate-buffered saline (PBS) and the other 3 groups injected with 5, 10, and 20 mg L-Met/egg, respectively. The injection was performed on embryonic day 9 (E9), and intestinal samples were collected on the day of hatching for analysis. The results showed that, compared with the CON group, the groups administered an in ovo injection of L-Met increased relative weights of the duodenum, jejunum, and ileum (P < 0.05). Hematoxylin and eosin (H&E) staining showed that the groups injected with 5, 10, and 20 mg L-Met significantly increased villus height and crypt depth (P < 0.05). Moreover, in ovo injection of 10 mg L-Met also increased the transepithelial electrical resistance (TEER) of the jejunum (P < 0.05). Injection with 10 and 20 mg L-Met increased the expression of the tight junction proteins (ZO-1 and claudin-1) and the fluorescence signal intensity of Ki67 and villin proteins (P < 0.05). Further, the protein expression of phospho-Janus kinase 2 (p-JAK2) and phospho-signal transducer and activator of transcription 3 (p-STAT3) was significantly increased by 10 or 20 mg L-Met injection (P < 0.05). In conclusion, the injection of L-Met, especially at a dose of 10 mg, showed beneficial effects on the intestinal integrity of chick embryos due to the activation of the JAK2/STAT3 signaling pathway. Our results may provide new insights for regulating the intestinal development of embryonic chicks and the rapid growth of chicks after hatching.
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DOI：
10.1158/1078-0432.ccr-10-2694
发表时间：
2011-03-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Morikawa T;Baba Y;Yamauchi M;Kuchiba A;Nosho K;Shima K;Tanaka N;Huttenhower C;Frank DA;Fuchs CS;Ogino S
通讯作者：
Ogino S
影响因子：
5.9
作者：
Richmond CA;Rickner H;Shah MS;Ediger T;Deary L;Zhou F;Tovaglieri A;Carlone DL;Breault DT
通讯作者：
Breault DT
影响因子：
5.6
作者：
Shillingford, Nick M.;Calicchio, Monica L.;Kozakewich, Harry P. W.
通讯作者：
Kozakewich, Harry P. W.
乳酸菌通过 LPL 分泌 IL-22 诱导 STAT3 信号通路激活，加速 ISC 再生，保护肠粘膜完整性
影响因子：
12.4
作者：
Hou, Qihang;Ye, Lulu;Yu, Qinghua
通讯作者：
Yu, Qinghua
Torc1的靶向破坏通过抑制繁殖鸽子（哥伦比亚利维亚）中农作牛奶蛋白的合成来抑制年轻的异类生长。
影响因子：
4.4
作者：
Chen MJ;Fu Z;Jiang SG;Wang XQ;Yan HC;Gao CQ
通讯作者：
Gao CQ